MicroSolv Technology Corporation

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catalog description

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printable Application Sheet

Open Tubular CEC Application Sheet

Benzodiazepines

Short Analysis Time
Simple, Easy, Fast
Cholesterol Coated Columns - High Surface Area

Method Conditions:
Voltage: 25kV
Capillary: MicroSolvCEC Cholesterol Coated Column for
CEC, 50µm x 70cm Long
Injection: 5 seconds @ 5"Hg, Hydrodynamic
Injection to Detector Length: 45cm
Run Buffer: 19mM Tris/30mM Phosphoric Acid
pH: 2.14
Detection: UV 254nm

PEAKS:

Temazepam
Oxazepam
Clonazepam
Diazepam
Nitrazepam

Discussion of ElectroChromatogram:
These analytes are highly basic compounds that are physiologically and commercially important. The highly symmetrical peaks for these compounds are evidence to the high selectivity and efficiency of the MicroSolvCEC Cholesterol Coated Column. The shoulders on peaks four and five are minor components.

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