Metallo-Proteins

• Short Analysis Time
• Simple, Easy, Fast
• Minimized Wall Adsorption

Method:
Performed on a Beckman P/ACE 5000 instrument with liquid cooling, Metallothionein samples of rabbit liver were purchased from Sigma Aldrich and prepared in ultra pure water at a concentration of 1.0mg/mL. Injection was performed using pressure and Methanol was added to the run buffer to reduce EOF and further minimize any wall adsorption. Capillaries were conditioned with run buffer for 30 minutes before flushing the capillaries with water for five minutes. Polarity was normal.

Discussion and Rationale:
Separation at slight alkaline pH was chosen due to the natural state of these proteins where the metals are still bound to molecules; at acidic pH the metals dissociate from the proteins. The CE100-SA Capillaries exhibited near pH independence and very minimal wall adsorption especially when Methanol was added. The E-gram shows rabbit liver MT-1 and MT-2 isoforms and sub-isoforms where all peaks are resolved. Difference in the polymorphisms of the rabbit liver can be seen.