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catalog description
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Open Tubular CEC Application Sheet
Serotonin and Tryptamine
- Short Analysis Time
- Simple, Easy, Fast
- Diol Coated Columns - High Surface Area
- C-18 Columns - High Surface Area
Method Conditions:
Voltage: 30kV
Capillary A: MicroSolvCEC Diol Coated Column for
OT-CEC, 50µm x 50cm
Capillary B: MicroSolvCEC C18 Coated Column for
OT-CEC, 50µm x 50cm
Injection: 5 seconds @ 5"Hg, Hydrodynamic
Length to Detector Window: 25cm
Run Buffer: 19mM Tris/30mM Phosphoric Acid, e-gram
A, 60mM Acetic Acid/60mM gamma-amino-butyric
acid. E-gram B.
pH: Chromatogram A: 4.41
Chromatogram B: 2.14
Detection: UV @223nm
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Discussion of ElectroChromatogram:
Tryptamine and Serotonin exist together in real samples and are very important physiologically. The
differences in the structure and charge to size ratio is very small; HPLC or CZE analysis is difficult.
Relatively quickly, these compounds can be resolved with high symmetry and high efficiency using OT-CEC.
The MicroSolvCEC C18 column allows good separation at pH 4.41 while the MicroSolvCEC Diol column allows
separation at low pH(2.1).
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