The MICROSOLV HSQ Kit is designed to help laboratories measure, document, and compare HPLC system performance using standardized qualification procedures and NIST-traceable reference materials. These sample results demonstrate the types of tests that can be performed as part of HPLC Performance Qualification (PQ), troubleshooting investigations, method transfer studies, instrument comparisons, and ongoing system performance monitoring.
The examples below illustrate actual test outputs generated using the HSQ Kit, including evaluations of autosampler precision, detector linearity, wavelength accuracy, gradient performance, extra-column volume, carryover, noise, sensitivity, and other critical system suitability parameters.
These results can help laboratories establish performance baselines, identify instrument-to-instrument differences, document compliance activities, and support data integrity initiatives across HPLC and UHPLC systems.
Autosampler Precision
Calculation of Extra-Column Volume
Detector Absorbance Linearity 100% - 0.1%
xtra Column Volume
Gradient Dwell Volume
radient Qualification Tests
Injector Percent Carry-Over
Primary Test Chromatogram
Sample Template Results
Short Term Dynamic Noise
Spectrum of Caffeine in RTM
Wavelength Accuracy from
Calibration Standard
Wavelength Accuracy – Primary Standard
Spectrum of NIST SRM 2034 (lot95) using an HP 8453 spectrophotometer (bandwidth 1nm). Official values are rounded to nearest nanometer. The bands with a * are recommended for use as calibration bands for HPLC and or CE detectors.
Effect of Extra Column Volume on Your Results
Below are two chromatogram examples of how two different instruments can produce very different results using the exact same conditions. They were both tested, using the exact same certified, HPLC Column, with the exact same NIST traceable standards on the exact same day. These differing results of an older and newer HPLC instrument may be simply they are plumbed quite differently.
Recommendation
: Make sure all fittings are new, secure and that all tubing is well cut and as short as possible. Not all HPLC instruments offer optimal extra volume; check with your instrument manufacturer on how to optimize your system.
Hint! Use the MICROSOLV™ PQ Kit™ for determining system, extra column volume before transferring methods to different instruments, departments or clients.