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Robust, Reproducible & Reliable EOF 

ORDERING INFORMATION

CElixir™ products are double layer, dynamic coating systems that will make your CZE more reproducible and produce results faster. CElixir™ makes CZE useful in the pH range of 1.0 to 9.2 in your lab with astonishing speed and precision. Unlike traditional CZE, the two ionic solutions of CElixir™ will produce very robust EOF allowing you to develop methods that are most optimal for your samples. You can select the pH where your analytes are most ionized at or below your samples pK. Improved selectivity, efficiency, speed and precision are now available to you with CZE. CElixir Dynamic Coatings are used in Research and in regulated GLP/GMP Pharmaceutical Laboratories.

How does it work?
The two poly-ionic solutions of CElixir™ are applied to a bare fused silica capillary producing a very stable polymeric coating on the wall. First the cationic, polymer of the Initiator Solution™ (A) binds to the ionized silanol sites of the capillary wall. Then the anionic polymer of the Accelerator Solution™ (B), with a vast excess of negative charge, is added. Thus, there is an increase in the negatively charged sites relative to the bare fused silica wall that is always fully charged. Most importantly, however, is that both the exposed polymer layer (negatively charged) and the base polymer layers are washed off between runs by a NaOH solution rinse. A virgin layer of both polymers is replaced before each analysis. This gives previously unheard-of reproducibility independent of source or age of the bare fused silica.

Two solutions of CElixir™ are placed in three vials on your HPCE instrument and are automatically applied to the capillary before and between runs. The CE practitioner is no longer relying on the fused silica capillary as the main component of the separation mechanism and EOF. The ionic polymers in CElixir™ now provide this separation mechanism, making it “portable”, controllable and reproducible. The solutions are always the same on any capillary you use and on any HPCE instrument that you use and therefore the EOF and CZE conditions are always the same anywhere any time.

Low pH, Yet High Flow Rate
Even at very low pH (as low as 1.7), CElixir™ has a very fast and reproducible EOF. If you change the pH from run to run, the resulting change in EOF speed will be negligible. This allows you to use pH as a solubility tool, a selectivity tool or as a sample matrix option to retain the biological activity of your samples. As the CElixir coatings are re-applied before each run minimizing the wall adsorption effects; the inherent capillary inconsistencies experienced at low pH are eliminated. Precision, speed and reproducibility are increased over normal Capillary Zone Electrophoresisce.

CElixir™ Kits: Description
CElixir-pH™ Full range of different Accelerator solutions to do your own method development throughout the pH range up to 9.2.
CElixir SDS™ Complete MEKC Kit which can be used to separate Neutral compounds or chiral compounds.
CElixirOA™ Our Most Popular CElixir. Complete Kit to separate Anions and Organic Acids.
CElixir-Plus™ Complete Kit to separate Cations including non UV adsorbing compounds.
CElixir-Pro™ Complete Coating Kit to separate some proteins and peptides.


 


   Features of CElixir™
   Benefits of CElixir™
Increases Precision of your EOF Quantify Peaks Easily & Reliably
Increases Reproducibility of your HPCE Increase Confidence In your Data
Can be Automated Easy to use, anyone in the lab can use it
pH Independent Use pH to control ionization of your sample & selectivity or maintain biological activity
Shifts Adsorption Equilibria Minimize Sample loss and Increase Reproducibility
Increases EOF Speed Shorter Analysis time, Increase lab throughput
Lower Cost v. HPLC Save Money buying solvents and disposal costs
No Buffer Preparation Needed Save Time and Increase Repeatability of Method
No Organics or Hazardous Material Used Solutions are stable and safe and easily disposed


 


More Information about CE & CElixir™ Dynamic Coatings:
Application Notes  (Knowledge Base) FAQ - General CE Tips  (Knowledge Base)
Download Literature - CElixirOA Brochure  (PDF) Ordering Information - CElixir
MicroSolv™ CE Primer - A Tutorial MSDS - Downloadable -  (PDF)
Product Manuals -  (PDF)  
CElixir Logo Graphic       

 

 

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