About CElixir™ Dynamic Coatings for CE - Reference Information

We regret to announce that this Product Line is discontinued as the End of February 2021 due to inability to obtain raw materials.

CElixir™ products are double layer, dynamic coating systems that will make your CZE more reproducible and produce results faster. CElixir™ makes CZE useful in the pH range of 1.0 to 9.2 in your lab with astonishing speed and precision. Unlike traditional CZE, the two ionic solutions of CElixir™ will produce very robust EOF allowing you to develop methods that are most optimal for your samples. You can select the pH where your analytes are most ionized at or below your samples pK. Improved selectivity, efficiency, speed and precision are now available to you with CZE. CElixir Dynamic Coatings are used in Research and in regulated GLP/GMP Pharmaceutical Laboratories.

How does it work?
The two poly-ionic solutions of CElixir™ are applied to a bare fused silica capillary producing a very stable polymeric coating on the wall. First the cationic, polymer of the Initiator Solution™ (A) binds to the ionized silanol sites of the capillary wall. Then the anionic polymer of the Accelerator Solution™ (B), with a vast excess of negative charge, is added. Thus, there is an increase in the negatively charged sites relative to the bare fused silica wall that is always fully charged. Most importantly, however, is that both the exposed polymer layer (negatively charged) and the base polymer layers are washed off between runs by a NaOH solution rinse. A virgin layer of both polymers is replaced before each analysis. This gives previously unheard-of reproducibility independent of source or age of the bare fused silica.

Two solutions of CElixir™ are placed in three vials on your HPCE instrument and are automatically applied to the capillary before and between runs. The CE practitioner is no longer relying on the fused silica capillary as the main component of the separation mechanism and EOF. The ionic polymers in CElixir™ now provide this separation mechanism, making it “portable”, controllable and reproducible. The solutions are always the same on any capillary you use and on any HPCE instrument that you use and therefore the EOF and CZE conditions are always the same anywhere any time.

Low pH, Yet High Flow Rate
Even at very low pH (as low as 1.7), CElixir™ has a very fast and reproducible EOF. If you change the pH from run to run, the resulting change in EOF speed will be negligible. This allows you to use pH as a solubility tool, a selectivity tool or as a sample matrix option to retain the biological activity of your samples. As the CElixir coatings are re-applied before each run minimizing the wall adsorption effects; the inherent capillary inconsistencies experienced at low pH are eliminated. Precision, speed and reproducibility are increased over normal Capillary Zone Electrophoresisce.