Famotidine Tablet Analyzed with HPLC - AppNote
August 12, 2012
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API Separation from Matrix Component
This Method for Analysis of Famotidine Tablets is easy to perform and produces a Symmetrical Peak Shape for the API. This compound has numerous amines which can be problematic in terms of Peak Shape with conventional Columns. Separation from a component from the tablet extract matrix is obtained as well, illustrating specificity of the Method.
Reproducibility is shown by the overlay of runs from two different Column lots.
Column: Cogent Diamond Hydride™, 4 μm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75 mm
Mobile Phase:
-- A: DI Water with 0.1% Trifluoroacetic Acid (TFA) v/v
-- B: Acetonitrile with 0.1% Trifluoroacetic Acid (TFA) v/v
Gradient:
Post Time: 3 minutes
Injection vol.: 1 μL
Flow rate: 1.0 mL / minute
Detection: UV @ 265 nm
Sample Preparation: 10 mg strength Famotidine tablet was ground and added to a 25 mL volumetric flask. A portion of 50:50 Solvent A / Solvent B diluent was added and the flask was sonicated 10 minutes. It was then diluted to mark and filtered with a 0.45 μm Nylon Syringe Filter (MICROSOLV Tech Corp.).
t 0 : 0.9 minutes
Attachment No 221 Famotidine Tablet Analyzed with HPLC pdf 0.4 Mb Download File
This Method for Analysis of Famotidine Tablets is easy to perform and produces a Symmetrical Peak Shape for the API. This compound has numerous amines which can be problematic in terms of Peak Shape with conventional Columns. Separation from a component from the tablet extract matrix is obtained as well, illustrating specificity of the Method.
Reproducibility is shown by the overlay of runs from two different Column lots.
Peaks:
1. Matrix
Component,
2. Famotidine
Column: Cogent Diamond Hydride™, 4 μm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75 mm
Mobile Phase:
-- A: DI Water with 0.1% Trifluoroacetic Acid (TFA) v/v
-- B: Acetonitrile with 0.1% Trifluoroacetic Acid (TFA) v/v
Gradient:
| Time (minutes) | %B |
|---|---|
| 0 | 95 |
| 2 | 95 |
| 6 | 50 |
| 7 | 95 |
Post Time: 3 minutes
Injection vol.: 1 μL
Flow rate: 1.0 mL / minute
Detection: UV @ 265 nm
Sample Preparation: 10 mg strength Famotidine tablet was ground and added to a 25 mL volumetric flask. A portion of 50:50 Solvent A / Solvent B diluent was added and the flask was sonicated 10 minutes. It was then diluted to mark and filtered with a 0.45 μm Nylon Syringe Filter (MICROSOLV Tech Corp.).
t 0 : 0.9 minutes
Attachment No 221 Famotidine Tablet Analyzed with HPLC pdf 0.4 Mb Download File