Ritonavir Analyzed with HPLC - AppNote
February 10, 2022
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Date: 10-FEBRUARY-2022 Last Update: 15-AUGUST-2026
A Reproducible Method for Analysis of a Protease InhibitorA rapid, sensitive, and Reproducible Method has been developed for this Antiretroviral Medication . The data below, (an overlay of 10 chromatograms ) illustrates how the compound can be adequately Retained and detected using this straightforward Method.
A Phenyl ring in the Column Stationary Phase provides strategic use of π-π Interaction with the Analyte making possible the use of a very simple, Mass Spec-friendly Mobile Phase with Formic Acid as an additive.
10 Injections of Ritonavir
Method Conditions
Column: Cogent Phenyl Hydride™, 4 μm, 100 Å
Catalog No.: 69020-10P
Dimensions: 4.6 x 100 mm
Mobile Phase: (65:35) Acetonitrile / DI Water with 0.1% Formic Acid
Injection vol.: 5 μL
Flow rate: 1.0 mL / minute
Detection: UV @ 254 nm
Sample Preparation: Ritonavir standard prepared as 1.0 mg / mL Standard Solution in Mobile Phase
t 0 : 1.20 Minutes
K: 1.2
Notes : Ritonavir was initially developed as an independent Antiviral Agent but has been shown to possess advantageous properties in combination regimens with low-dose Ritonavir and other Protease Inhibitors. Currently, it is more commonly used as a booster of other Protease Inhibitors.
