Prep HPLC Column, NP Amino, Wide Pore, 10um, 300A. 21.2mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent NP Amino is a versatile normal phase and HILIC stationary phase designed for retention of highly polar compounds that are often difficult to retain using conventional reversed-phase columns. Manufactured on high-purity silica with a propyl amino bonded phase, it provides excellent selectivity for carbohydrates, sugars, organic acids, and other polar analytes. This stationary phase is commonly used as an alternative for USP L8 methods and is suitable for both Normal Phase and HILIC separations.
68040-P21-250 is a preparative HPLC column packed with 10 µm NP Amino particles featuring a 100 Å pore size in a 21.2 mm ID x 250 mm format. The large internal diameter provides substantial sample loading capacity for purification workflows, while the 250 mm length maximizes resolving power and peak capacity for demanding preparative separations. The 10 µm particle size supports higher flow rates with lower operating backpressure, making it well suited for preparative chromatography and scale-up applications. Typical operating flow rates are approximately 15–40 mL/min depending on sample load, mobile phase composition, and system capabilities.
This column is particularly effective for sugars, carbohydrates, negatively charged compounds, organic acids, carboxylic acid-containing compounds, and other highly polar molecules. As with all amino phases, this column is suitable for both Normal Phase and HILIC applications when enhanced retention of polar analytes is required.
Key Benefits
NP Amino stationary phase
USP L8 equivalent chemistry
Propyl amino bonded phase
High-purity silica support
Suitable for Normal Phase chromatography
Suitable for HILIC applications
Enhanced retention of polar compounds
Excellent for sugars and carbohydrates
Low operating backpressure with 10 µm particles
Maximum loading capacity and resolution for preparative purification
Typical Applications
Preparative chromatography
USP L8 methods
HILIC separations
Normal Phase chromatography
Sugar purification
Carbohydrate purification
Organic acid analysis and purification
Carboxylic acid separations
Pharmaceutical purification
Method scale-up from analytical columns
Specifications
Product Type: Preparative HPLC Column
Stationary Phase: NP Amino
USP Classification: L8
Chromatography Mode: Normal Phase / HILIC
Bonded Phase: Propyl Amino
Endcapping: Not Endcapped
Particle Size: 10 µm
Pore Size: 100 Å
Column Dimensions: 21.2 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 15–40 mL/min
Brand: Cogent
Special Order Item Information
Important Notes
Packed and shipped in Normal Phase solvents
Suitable for both Normal Phase and HILIC chromatographic modes
Excellent retention of sugars, carbohydrates, and organic acids
Extended column length maximizes resolution and peak capacity
Ideal for preparative purification and scale-up applications
Requires a preparative HPLC system capable of operating at approximately 15–40 mL/min
This is a Special Order Item