Process HPLC Column, NP Amino, Wide Pore, 10um, 300A. 50mm ID x 50mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent NP Amino Wide Pore is a versatile normal phase and HILIC stationary phase designed for retention of highly polar compounds and larger molecules that are often difficult to retain using conventional reversed-phase columns. Manufactured on high-purity 300 Å wide pore silica with a propyl amino bonded phase, it provides excellent selectivity for complex carbohydrates, sugars, organic acids, and other highly polar analytes. This stationary phase is commonly used as an alternative for USP L8 methods and is suitable for both Normal Phase and HILIC separations involving larger molecular species.
68040-P50-50-3M is a process-scale HPLC column packed with 10 µm NP Amino Wide Pore particles featuring a 300 Å pore size in a 50 mm ID x 50 mm format. The large internal diameter provides substantial sample loading capacity for purification, production, and scale-up workflows, while the short 50 mm length is optimized for rapid process development, production screening, and scale-up studies. The 10 µm particle size supports high flow rates with relatively low operating backpressure, while the wide pore structure improves accessibility for larger molecules. Typical operating flow rates are approximately 80–200 mL/min depending on sample load, mobile phase composition, and system capabilities.
This column is particularly effective for complex sugars, carbohydrates, negatively charged compounds, organic acids, carboxylic acid-containing compounds, and other highly polar molecules. The wide pore design provides improved performance for larger analytes in both Normal Phase and HILIC applications. As with all amino phases, this column is suitable for HILIC applications when enhanced retention of polar compounds is required.
Key Benefits
NP Amino Wide Pore stationary phase
USP L8 equivalent chemistry
Propyl amino bonded phase
300 Å wide pore silica
Suitable for Normal Phase chromatography
Suitable for HILIC applications
Enhanced retention of polar compounds
Improved accessibility for larger molecules
Low operating backpressure with 10 µm particles
Ideal for process development and scale-up studies
Typical Applications
Process-scale chromatography
Purification and production workflows
USP L8 methods
HILIC separations
Normal Phase chromatography
Complex sugar purification
Carbohydrate purification
Organic acid purification
Pharmaceutical manufacturing and scale-up
Process development
Specifications
Product Type: Process HPLC Column
Stationary Phase: NP Amino Wide Pore
USP Classification: L8
Chromatography Mode: Normal Phase / HILIC
Bonded Phase: Propyl Amino
Endcapping: Not Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 50 mm ID x 50 mm
Length: 50 mm
Typical Flow Rate: 80–200 mL/min
Brand: Cogent
Special Order Item Information
Important Notes
Packed and shipped in Normal Phase solvents
Suitable for both Normal Phase and HILIC chromatographic modes
Wide pore design improves access for larger molecules and complex carbohydrates
Excellent retention of sugars, carbohydrates, and organic acids
Short column length is ideal for rapid process development and scale-up studies
Ideal for process-scale purification and production applications
Requires a specialized process-scale HPLC system capable of operating at approximately 80–200 mL/min
This is a Special Order Item