Catalog Number - 62318-15P-2

Hyper Similars C18™ is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped octadecyl (C18) bonded phase. Designed as a cost-effective alternative to legacy Hypersil® and Spherisorb® columns, it offers comparable chromatographic behavior for USP L1 methods while providing the strong hydrophobic retention and reproducibility expected from a conventional C18 stationary phase.

62318-15P-2 is a 150 mm x 2.1 mm ID narrow-bore analytical column packed with 3 µm, 150Å Hyper Similars C18 particles. The 150 mm length provides increased resolving power compared to shorter columns, while the 2.1 mm ID format minimizes solvent consumption and is optimized for LC-MS applications. The 3 µm particle size delivers higher efficiency and sharper peak shapes than comparable 5 µm packings, although higher backpressure should be expected. Typical operating flow rates are approximately 0.2–0.3 mL/min and require a system capable of reliable low-flow operation and low-volume flow path design.

This USP L1 column is ideal for hydrophobic and neutral compounds when greater separation is desired in LC-MS methods. Common applications include pharmaceutical analysis, bioanalytical assays, long-chain fatty acid separations, and method development where high sensitivity and efficient chromatographic performance are important.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L1 methods

• Comparable selectivity to Hypersil® and Spherisorb® C18 columns

• Narrow-bore format ideal for LC-MS applications

• Increased resolution from 150 mm column length

• Higher efficiency with 3 µm particles

• Reduced solvent consumption

• End-capped C18 bonded phase

Typical Applications

• USP L1 methods

• LC-MS analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Bioanalytical methods

• Long-chain fatty acid analysis

• Method development

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Hyper Similars C18

• USP Classification: L1

• Particle Size: 3 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C18 (Octadecyl)

• Endcapped: Yes

• Pore Size: 150Å

• Column Dimensions: 2.1 mm ID x 150 mm

• Length: 150 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Narrow-bore format optimized for LC-MS applications

• Requires an HPLC or LC-MS system capable of reliable low-flow operation

• 3 µm particles provide higher efficiency and sharper peaks

• Higher backpressure than comparable 5 µm columns

• Reduced solvent consumption compared to 4.6 mm ID columns

• Increased resolving power for complex separations

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