Select a HILIC column phase Above or Below based on your analyte chemistry and selectivity requirements.
These columns provide strong, reproducible retention of polar and highly functional compounds while avoiding the equilibration and stability issues common with conventional HILIC materials. Ideal for LC‑MS workflows, metabolites, sugars, and difficult polar analytes.
Columns for Polar Compounds in HILIC Methods
These HILIC columns are designed for the effective retention and separation of polar, highly functional, and water‑soluble compounds that are poorly retained or unresolved by traditional reversed‑phase HPLC. HILIC methods are widely used for the analysis of small polar molecules, sugars, amino acids, metabolites, and other challenging analytes, particularly in LC‑MS and LC‑MS/MS workflows.
These HILIC columns are engineered to provide stable retention, fast equilibration, and reproducible performance across a broad range of mobile phase conditions. These columns help eliminate common HILIC frustrations such as long conditioning times, retention variability, and sensitivity to minor method changes, making them suitable for both routine analytical methods and advanced method development.
Key Benefits of Cogent HILIC Columns
Strong and predictable retention of polar compounds
Improve resolution and peak shape for analytes that elute near the void on reversed‑phase columns.
Fast equilibration and method robustness
Reduce downtime between injections and improves run‑to‑run consistency, even during gradient elution.
LC‑MS friendly performance
Designed for high organic mobile phases commonly used in mass spectrometry, supporting sensitivity and reproducibility.
Broad applicability across industries
Suitable for pharmaceutical, bioanalytical, clinical, food, and research laboratories.
Column Phases Available in This Category
This HILIC methods category includes multiple stationary phases that offer different selectivity profiles, allowing users to fine‑tune separations based on analyte chemistry: Each phase offers a distinct interaction mechanism for optimizing retention, resolution, and selectivity. For most polar LC‑MS applications, Diamond Hydride HILIC columns are a common starting point.
Core HILIC Column Phases:
Method Development Tools:
Each phase provides a distinct interaction mechanism, enabling optimization of retention, resolution, and selectivity without changing separation mode.
When to choose HILIC for Method Development
HILIC methods are typically selected when:
Polar analytes show little or no retention in reversed‑phase HPLC
Early‑eluting compounds require improved resolution
LC‑MS methods benefit from high organic mobile phases
Consistent retention of polar compounds is critical for quantitation.
