HPLC Troubleshooting and Assistance Form - MicroSolv Technology Corp

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HPLC Troubleshooting and Assistance Form

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Issues:

Separation Goals:
State what your goals are for this separation that you are having trouble with. Be brief but specific. Please include any specific requirements you have such as alpha values?

Samples:
Provide as much information as possible including pI or pKa of each molecule, molecular weight and sample matrix. Identify which analytes are major or minor components.

Storage Requirements:
What if any, storage requirements do these Samples have?

Solubility of Samples:
What is the solubility of the samples in the defined injection solvent and its stability when made. Be brief and specific.

Detection Limits:
What detection type are you using and what is the detection limit if known? If using MS, is it MS Only? If yes, MH+= and MH-=?

Buffers:
Is your mobile phase method buffered? What buffers are you using and what is the Molarity?
Was the pH adjusted before or after the organic solvents were added?
Did you equate buffers in both solvents? Can you change the buffer or mobile phase pH of choice?
Can you change the ratio of solvents in the mobile phase?

Temperature:
What temperature did you use?
Did you use temperature for stability, backpressure, speeding up analysis or to enhance selectivity?

Chromatogram/E-Gram:
Please state in which way you would like to improve your current chromatogram/electropherogram. What if any acceptance criteria do you have for Peak Shape?

HPLC/CE Instrumentation Used:
Which system was used for the current chromatogram/electropherogram?
What is the dead volume from pump start to gradient seen in detector (for HPLC only).

For HPLC, which HPLC Columns or CE Capillaries were used?:
Please state stationary phase, pore size, particle size, column length and width, brand and age of column currently used. For CE, which capillaries were used: include length, ID, coatings if any, and distance from window to detector.
Are you free to change stationary phase or column specifications?
What limits are there on the columns you can use?

What conditions did you use already and which columns did you use with these conditions?:
Are you looking for an improvement in the existing method or do you not have any separation yet?

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