Catalog Number - 68334-10P-2

Cogent RP Cyano™ is a versatile reversed-phase HPLC stationary phase designed for the separation of polar and neutral compounds. Manufactured using a three-carbon ligand with a terminal cyano (CN) functional group bonded to ultra-high purity silica, this stationary phase provides selectivity distinct from traditional C18, C8, and phenyl phases. As a USP L10 equivalent phase, it is widely used for method replacement, method development, and applications requiring reduced hydrophobic retention, faster equilibration, and alternative selectivity.

68334-10P-2 is an HPLC column packed with 3 µm RP Cyano™ particles featuring a 100 Å pore size in a 2.1 mm ID x 100 mm format. This popular narrow-bore configuration combines enhanced chromatographic resolution with reduced solvent consumption and excellent compatibility with LC-MS instrumentation. The 100 mm length provides greater resolving power than shorter 50 mm columns, while the 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns. Typical operating flow rates are approximately 0.2–0.3 mL/min. Because of the low flow rates required by 2.1 mm ID columns, standard HPLC systems must be capable of precise and reproducible low-flow operation.

Often selected for polar and neutral compounds in reversed-phase HPLC and LC-MS methods, this Cyano phase typically provides shorter retention times and faster equilibration than non-modified silica columns. As one of the most popular RP Cyano™ formats, it is frequently used for USP L10 methods, pharmaceutical analyses, and LC-MS applications requiring increased resolution and alternative selectivity.

Key Benefits

• RP Cyano™ stationary phase

• USP L10 equivalent chemistry

• Cyano bonded phase

• Ultra-high purity silica support

• Alternative selectivity to C18 and C8 phases

• Faster equilibration between runs

• Shorter retention for many compounds

• 3 µm particle size

• Greater efficiency than 5 µm columns

• Excellent for LC-MS applications

Typical Applications

• USP L10 methods

• LC-MS methods

• Polar compound analysis

• Neutral compound analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Method development

• Quality control testing

• Alternative selectivity studies

• Stability testing

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP Cyano™

• USP Classification: L10

• Chromatography Mode: Reversed Phase

• Bonded Phase: Cyano (CN)

• Endcapping: Not End Capped

• Particle Size: 3 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 100 mm

• Length: 100 mm

• Typical Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase HPLC and LC-MS applications

• Provides alternative selectivity compared to C18, C8, and Phenyl phases

• Suitable for polar and neutral compounds

• Faster equilibration than many traditional reversed-phase columns

• The 2.1 mm ID format is commonly used for LC-MS applications

• Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.3 mL/min

• Detector flow cells should be optimized for low-volume, narrow-bore chromatography

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• Samples should be filtered with a 0.2 µm syringe filter before injection

• Packed and shipped in reversed-phase solvents

• For normal-phase applications, see the related Cogent NP Cyano Columns packed in normal-phase solvents