Equation:

RS = 2(t R2 -t R1 /W 2 +W 1 )

  • tR2 = Retention Time of Your Late Eluting Peak (Shorter Time)
  • tR1 = Retention Time of Your Early Eluting Peak (Longer Time)
  • PW2 = Width of Your Late Eluting Peak (Shorter Time)
  • PW1 = Width of Your Early Eluting Peak (Longer Time)

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