Glass Vacuum Filtration & Degassing Assemblies for HPLC

All‑glass vacuum filtration and degassing assemblies are used for bulk preparation of HPLC mobile phases and buffers, combining particulate removal and solvent degassing in a single, integrated workflow. This category includes borosilicate glass filtration assemblies designed for use with membrane filter discs during mobile‑phase preparation.

Vacuum filtration and degassing using all‑glass assemblies has long been a standard practice in liquid chromatography, particularly in laboratories that prepare solvents in‑house and require reliable, low‑contamination workflows.

Design & Construction

These assemblies are constructed entirely from borosilicate glass, providing:

• Chemical resistance to a wide range of aqueous and organic solvents

• Compatibility with aggressive mobile‑phase chemistries

• Dimensional stability during vacuum‑assisted operation

Typical assemblies include a glass funnel, fritted glass membrane support, and vacuum flask reservoir, forming a closed system suitable for filtration and degassing under reduced pressure.

Filtration & Degassing Function

When used with appropriate membrane discs, all‑glass vacuum assemblies:

• Remove particulates from mobile phases prior to system introduction

• Assist in the removal of dissolved gases during vacuum application

• Support preparation of solvents and buffers used in HPLC, UHPLC, and LC‑MS workflows

Filtration performance depends on membrane material and pore size selection, which should be matched to solvent composition and analytical sensitivity.

Membrane Compatibility

These assemblies are compatible with standard 47 mm membrane discs, typically with pore sizes ≥ 0.2 µm, allowing laboratories to select membranes optimized for:

• Aqueous or organic solvents

• Low‑extractables requirements

• LC‑MS or UHPLC applications

Membrane selection directly influences system cleanliness and analytical reliability.

Performance & Use Considerations

• Bulk volumes of solvents or buffers may be prepared efficiently

• Fritted glass membrane supports help prevent particulate contamination of vacuum sources

• Proper selection of vacuum pumps and operating conditions is recommended to ensure safe use with glass assemblies

These systems are intended for controlled laboratory environments and standard chromatography solvent preparation workflows.

Applications

All‑glass vacuum filtration and degassing assemblies are commonly used for:

• HPLC and UHPLC mobile‑phase preparation

• Buffer filtration prior to chromatographic analysis

• Analytical and regulated laboratory workflows

• In‑house solvent preparation programs

Related Categories

• Membrane Discs for Mobile‑Phase Filtration

• Mobile‑Phase Inlet Filters

• Inline Filters for HPLC & LC‑MS