Catalog Number - 58022-PES25-C

The 58022-PES25-C AQ™ Syringe Filters are pharmaceutical-grade, high-performance membrane filters designed for HPLC sample preparation, sterile filtration, and dissolution testing in regulated laboratory environments. Featuring a 0.22 µm Polyethersulfone (PES) membrane, these filters deliver exceptional consistency, ultra-low protein binding, and extractable-free performance, ensuring maximum sample purity and reproducible analytical results.

The 0.22 µm pore size is optimized for sterile filtration and fine particulate removal, making these filters ideal for biological samples, buffers, and protein-containing solutions where high recovery and cleanliness are critical.

✅ Critical Compliance Advantage (No Flush Required)

These filters are validated and certified to meet stringent regulatory requirements, including:

• US FDA CFR Title 21, Part 211.92 for minimum fiber release

• USP <788> for particulate matter in injectables

• USP requirements for “Sterile Water for Injection”

Because of this validated performance:

• ✅ No initial flushing is required

• ✅ No need to discard the first 200 µL of filtrate

• ✅ Preserves valuable samples (especially critical for protein recovery)

• ✅ Eliminates a common source of failed methods and investigations

👉 This provides a critical operational and compliance advantage for QC/QA laboratories, particularly in biopharmaceutical and sterile filtration workflows.

Performance & Design

The 0.22 µm PES membrane provides reliable sterile filtration with ultra-low protein binding, ensuring maximum recovery of biomolecules and sensitive analytes.

PES membrane chemistry offers excellent flow rates, low extractables, and minimal adsorption, making it ideal for protein solutions, culture media, and buffer systems.

These filters deliver absolute retention and low hold-up volume, supporting accurate quantitation and minimal sample loss, even in demanding workflows.

The 25 mm format supports higher sample volumes and high-throughput workflows, while the white housing with red lettering allows quick identification of 0.22 µm PES filters.

Each pack includes a Certificate of Analysis, ensuring full traceability and compliance.

Key Features

0.22 µm PES Membrane (Pharma Grade)

• Ideal for sterile filtration and fine particulate removal

• Ensures maximum sample clarity

✅ No Initial Flush Required (Validated)

• Extractable-free from first filtrate

• No 200 µL discard required

Ultra-Low Protein Binding

• Maximizes recovery of proteins and biomolecules

• Ideal for biological and biopharma samples

High Throughput Performance

• Fast filtration with minimal resistance

• Suitable for high-volume workflows

Low Extractables

• Supports clean chromatography

• Improves reproducibility

Absolute Retention

• Reliable sterile filtration performance

• Suitable for critical analytical methods

Regulatory Compliance

• Meets FDA CFR 21 (211.92) requirements

• Complies with USP <788>

• Suitable for Sterile Water for Injection

25 mm Diameter for High Throughput

• Handles larger sample volumes

• Improves lab productivity

Autoclavable & EO Sterilizable

• 121°C for 30 minutes or Ethylene Oxide

Color-Coded (White / Red)

• Identifies 0.22 µm PES filters

Typical Applications

• HPLC & UHPLC Sample Preparation – fine particulate removal prior to injection

• Sterile Filtration – bacterial and contaminant removal

• Protein & Biomolecule Filtration – low binding, high recovery

• Cell Culture Media & Buffers – clean and efficient filtration

• Dissolution Testing – pharmaceutical QC workflows

• Biopharmaceutical Analysis – sensitive sample handling

• Regulated Laboratory Methods – validated, compliant processes

Specifications

• Filter Type: Syringe Filter (Pharma Grade)

• Membrane Material: PES (Polyethersulfone)

• Pore Size: 0.22 µm

• Diameter: 25 mm

• Housing Material: Polypropylene (PP)

• Color Code: White / Red (0.22 µm PES)

• Connection Type: Luer Lock / Slip

• Chemical Compatibility: Aqueous solutions, buffers, biological samples

• Protein Binding: Ultra-low

• Extractables: None detectable (no flush required)

• Sterilization: Autoclave or EO

• Compliance: FDA CFR 21 (211.92), USP <788>

• Certification: Certificate of Analysis included

• Sterility: Non-sterile

• Pack Size: 100/PK

• Brand: AQ™

Resources & Ordering Options

• Order PES syringe filters, 0.22 µm, 25 mm – 1000/case quantity

• Purchase PES syringe filters, 0.22 µm, 25 mm – 50/pack quantity