HPLC Column, Silica-C, 4um, 2.1mm ID x 100mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑10P‑2 Silica‑C™ HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering distinct selectivity and robust performance for HILIC and normal‑phase separations. Unlike conventional bonded‑silica phases, this unmodified silica hydride surface contains no end‑capping or organic ligands, resulting in stable retention behavior and rapid equilibration—particularly beneficial in moisture‑sensitive normal‑phase workflows.
This column is optimized for analytes requiring non‑aqueous or minimally aqueous mobile phases, where traditional silica can present variability due to moisture adsorption.
Application Focus: Silica‑C™ for HILIC & Normal‑Phase Separations
Silica‑C™ stationary phases are selected for the analysis of compounds that are not soluble in water or require non‑polar solvent systems. The Silica‑C™ phase is particularly effective for:
Lipids
Peptides and proteins
Oligonucleotides
Other analytes requiring non‑polar solvents or HILIC conditions
The silica hydride surface supports reproducible retention and improved method stability compared to conventional silica phases.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica columns, Silica‑C™ columns offer:
Faster equilibration between injections
No need to control moisture in mobile‑phase solvents
Robust column lifetime under demanding solvent conditions
Unique selectivity, especially valuable in normal‑phase separations
These attributes make Silica‑C™ a dependable choice for consistent, non‑aqueous analytical workflows.
Why Choose the 2.1 mm × 100 mm Format
The 2.1 mm ID × 100 mm length configuration is frequently selected when increased resolution and sensitivity are required, particularly for LC‑MS applications:
Higher resolving power than 50 mm formats
Improved peak capacity for complex sample mixtures
Reduced solvent consumption compared to larger‑diameter columns
Enhanced LC‑MS compatibility due to reduced flow to the MS source
This size offers a practical balance between resolution, run time, and solvent efficiency.
Particle Size and Performance
The 4 µm particle size provides reliable efficiency and consistent peak shape with manageable backpressure, supporting robust performance on standard analytical HPLC systems while delivering meaningful resolution improvements over shorter formats.
System Requirements and Flow Rates
Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min
System capability: Requires an HPLC system capable of accurate, stable low‑flow delivery
Important note: Not all standard HPLC systems are suitable for reliable operation at these reduced flow rates
Flow‑rate optimization should consider mobile‑phase viscosity, column temperature, and system pressure limits.