Prep HPLC Column, Bidentate C18, 4um, 100A. 30mm ID x 250mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40018‑P30‑250 Cogent Bidentate C18™ Preparative HPLC Column is the largest‑diameter and longest‑length option in the Bidentate C18™ lineup, engineered for maximum loading capacity, extended retention, and the highest achievable preparative resolution. This column is specified when purification workflows demand full separation of closely related compounds at high sample mass, prior to fraction collection.
Manufactured using Cogent TYPE‑C™ silica hydride technology, the column delivers excellent selectivity, rapid equilibration, and extremely robust column lifetime under aggressive preparative conditions. The stationary phase consists of an aliphatic C18 chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This chemistry creates a phase that is highly hydrophobic yet unusually stable, supporting purification methods that involve aqueous‑dominant mobile phases or low‑pH conditions—environments where conventional silica‑based prep C18 columns may degrade or lose performance.
With a 30 mm internal diameter and an extended 250 mm length, this column provides the highest plate count available in a 30 mm preparative format, making it the preferred choice for structurally similar impurities, isomers, or difficult separations where fraction purity is critical.
System Note: A specialized preparative HPLC system is required, including high‑flow pump capacity, appropriate tubing and valving, compatible injectors, and fraction‑collection hardware designed for large‑diameter columns.
Why Choose the 30 × 250 mm Prep Bidentate C18™ Format
This configuration is typically selected when:
Maximum preparative resolution is required
Shorter prep columns (50–150 mm) fail to resolve critical impurities
Very large sample loads must be purified with tight fraction cuts
Hydrophobic or basic (amine‑containing) analytes challenge standard prep C18 phases
The extended length dramatically increases interaction time and selectivity, while TYPE‑C™ silica hydride chemistry maintains fast equilibration and long service life even under the most demanding purification protocols.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Non‑traditional reversed‑phase surface chemistry
Faster equilibration than conventional preparative C18 columns
Extremely robust lifetime for repeated prep runs
Bidentate C18™ Bonding
Two‑point attachment via direct Si–C bonds
Strong, stable hydrophobic retention
Particularly effective for hydrophobic and amine‑containing analytes
Preparative Geometry for Maximum Resolution
30 mm ID × 250 mm length
Highest plate count in the 30 mm prep family
Enables full resolution of closely eluting components
Broad Operating Window
Compatible with aqueous‑dominant mobile phases
Stable under low‑pH conditions
Tolerates aggressive, high‑load preparative methods
Typical Applications
Large‑scale preparative purification
Resolution of closely related or isomeric compounds
Hydrophobic molecule and metabolite isolation
Amine‑containing analytes
Scale‑up from analytical, semi‑prep, or shorter prep columns
Workflows requiring the highest possible fraction purity
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable