HPLC Column, Diol, 4um, 4.6mm ID x 50mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40060‑05P Diol HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, providing excellent selectivity, faster equilibration, and robust column lifetime compared to conventional bonded‑silica diol phases. The 4 µm silica hydride particles offer dependable chromatographic performance with moderate backpressure, making this column well suited for routine analytical and high‑throughput workflows.
The TYPE‑C™ surface chemistry supports stable retention behavior across reversed‑phase (RP), normal‑phase (NP), and HILIC methods, enabling flexible method development without compromising reproducibility.
Application Focus: Diol Chemistry for HILIC, NP & RP
Diol stationary phases are commonly selected for the analysis of polar compounds that are difficult to retain by reversed‑phase chromatography. This column performs effectively for a wide range of analytes, including:
Polar and non‑polar peptides
Amino acids
Polar metabolites
Small biological and biomolecular compounds
The balanced polarity of the Diol phase allows seamless operation across HILIC, NP, and RP modes, making it a versatile choice for analytical laboratories.
Cogent TYPE‑C™ Silica Hydride Advantages
Compared with conventional bonded‑silica Diol columns, the TYPE‑C™ silica hydride platform delivers measurable operational benefits:
Faster equilibration, particularly in HILIC methods
Excellent selectivity for polar and mixed‑polarity analytes
Improved durability and column lifetime
Reduced susceptibility to silica hydrolysis, extending usable life
These properties make the 40060‑05P well suited for repeated use in high‑throughput and screening environments.
Why Choose the 4.6 mm × 50 mm Format
The 4.6 mm internal diameter is optimized for robustness, throughput, and compatibility with standard HPLC systems, making it an ideal choice for routine and screening applications:
Good resolution with short run times
Higher sample loading capacity than narrow‑bore formats
Excellent compatibility with autosamplers and standard detectors
Reliable performance in high‑throughput screening workflows
This column size is widely adopted in QC and screening laboratories where speed, repeatability, and ease of use are priorities.
Particle Size and Performance
The 4 µm particle size strikes a practical balance between efficiency and robustness, offering:
Consistent chromatographic performance
Lower pressure requirements
Broad system compatibility
While not intended for maximum efficiency LC‑MS optimization, this format excels in routine analytical and screening‑focused applications.
System Requirements and Flow Rates
Typical flow range (4.6 mm ID): ~0.8–1.2 mL/min
System compatibility: Designed for standard analytical HPLC systems
Operational note: Flow rates may be adjusted based on mobile‑phase viscosity, temperature, and pressure limits
This column does not require specialized low‑flow pumps or configurations, supporting straightforward integration into existing methods.