Prep HPLC Column, Diol, 4um, 100A. 21.2mm ID x 100mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40060‑P21‑100 Diol Preparative HPLC Column is designed for high‑capacity preparative purification workflows where semi‑preparative formats no longer provide sufficient resolving power or loading capability. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column enables efficient isolation and collection of milligram‑to‑gram‑level quantities of polar and amine‑containing compounds while preserving the selectivity and retention behavior inherent to Diol chromatography.
The increased column length provides enhanced resolution and peak capacity, making this format suitable for more challenging preparative separations.
Preparative Use Case: Why 21.2 mm ID × 100 mm
The 21.2 mm internal diameter classifies this column as full preparative, enabling high‑load purification and large‑volume fraction collection. Compared to 10 mm semi‑preparative columns, this format:
Supports substantially higher sample loading
Enables large‑volume fraction collection
Preserves chromatographic selectivity during scale‑up
Bridges pilot purification and routine preparative workflows
The 100 mm column length provides increased resolving power relative to 50 mm formats, improving separation of closely eluting polar analytes while maintaining practical throughput.
Diol TYPE‑C™ Stationary Phase Advantages
Manufactured using Cogent TYPE‑C™ silica hydride technology, the Diol stationary phase delivers key advantages for preparative HILIC and normal‑phase separations:
Excellent selectivity for polar and amine‑containing compounds
Faster equilibration than conventional bonded‑silica Diol phases
Robust column lifetime, even under high‑organic mobile‑phase conditions
This chemistry is particularly effective for purification of:
Polar small molecules
Metabolites
Analytes containing amine or basic functional groups
Compounds poorly retained by reversed‑phase methods
Made‑to‑Order Columns & Pricing Information
Preparative columns are application‑specific tools and represent a significant investment. To ensure correct selection and system compatibility:
Columns are manufactured to order based on customer requirements
Orders cannot be returned or canceled once placed
Pricing and technical guidance are provided by Customer Service via formal quotation
Please contact Customer Service using our online form or by phone to initiate the quotation process.
System Requirements
Typical operating flow rates: ~10–30 mL/min
Requires a preparative HPLC system capable of:
High‑flow solvent delivery
Large injection volumes
Appropriate detectors and fraction‑collection hardware
Standard analytical and semi‑preparative HPLC systems are not suitable for 21.2 mm ID preparative columns.
Flow rates should be optimized based on mobile‑phase viscosity, system pressure limits, and purification objectives.