Catalog Number - 40218-10P-2

The 40218‑10P‑2 Cogent Bidentate C18™ 2.o HPLC Column is a high‑efficiency, narrow‑bore analytical column designed for workflows that require greater resolving power than short‑length formats while preserving LC‑MS sensitivity. Built on Cogent TYPE‑C™ silica hydride technology, this column combines 2.2 µm particles with bidentate C18 bonding to deliver excellent selectivity, faster equilibration, and near‑UHPLC performance on standard HPLC instruments.

The Bidentate C18™ 2.o stationary phase is particularly effective for hydrophobic and very non‑polar compounds that are difficult to retain in aqueous mobile phases. Compared to conventional silica‑based C18 columns, the TYPE‑C™ hydride surface chemistry reduces secondary interactions, supporting more stable retention and improved peak shape under a wide range of operating conditions.

With a 2.1 mm internal diameter and an extended 100 mm length, this column provides increased plate count and separation efficiency relative to 50 mm formats, making it one of the most popular 2.o column sizes for LC‑MS when additional resolution is desired.

Why Choose the 2.1 × 100 mm Bidentate C18™ 2.o Format

This column size is commonly selected when:

• LC‑MS methods require higher resolution than 50 mm columns provide

• Closely eluting hydrophobic analytes must be better separated

• Near‑UHPLC efficiency is desired without upgrading to UHPLC instrumentation

• Retention stability is required under aqueous‑dominant or low‑pH methods

Combined with TYPE‑C™ silica hydride chemistry, the 100 mm length increases resolving power while maintaining fast equilibration and long column life.

Key Features

Cogent TYPE‑C™ Silica Hydride Technology

• Reduced silanol activity and secondary interactions

• Faster equilibration than conventional silica‑based C18 columns

• Excellent chemical robustness and reproducibility

Bidentate C18™ 2.o Bonding

• Two‑point attachment via direct Si–C bonds

• Strong hydrophobic retention with controlled selectivity

• Effective for very non‑polar compounds

Near‑UHPLC Efficiency (2.2 µm)

• Increased efficiency compared with 3–5 µm columns

• High performance on standard HPLC systems

• Improved peak capacity and resolution

LC‑MS‑Optimized Geometry

• 2.1 mm ID enhances sensitivity and reduces solvent use

• 100 mm length provides higher resolving power

• Well suited for ESI‑MS and LC‑MS/MS workflows

System Requirements

Due to the narrow‑bore, small‑particle format, optimal performance requires:

• A pump capable of precise, stable low‑flow delivery

• A detector flow cell designed for reduced internal volume

Systems not designed for low‑flow operation may not achieve full efficiency.

Typical Applications

• LC‑MS and LC‑MS/MS

• Hydrophobic and very non‑polar compounds

• Methods requiring increased resolution

• Near‑UHPLC analytical workflows

• Method development and validation