HPLC Column, Bidentate C18 2.o, 2.2um, 2.1mm ID x 150mm Length, 120A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40218‑15P‑2 Cogent Bidentate C18™ 2.o HPLC Column is a high‑efficiency, long‑format narrow‑bore column designed for separations where maximum resolution and near‑UHPLC performance are required. Built on Cogent TYPE‑C™ silica hydride technology, this column combines smaller 2.2 µm particles with bidentate C18 bonding to deliver increased efficiency, excellent selectivity, and fast equilibration—while remaining compatible with standard HPLC instrumentation.
The silica hydride surface, coupled with direct silicon–carbon bonds, provides exceptional chemical robustness and reduced secondary interactions compared with conventional silica‑based C18 columns. The Bidentate C18™ 2.o phase is especially effective for very hydrophobic and non‑polar compounds that are difficult to retain under aqueous mobile‑phase conditions.
With a 2.1 mm internal diameter and an extended 150 mm length, this column offers the highest plate count in the family, making it the preferred option when increased resolution is essential. It can be used for LC‑MS applications, but requires instrumentation capable of precise low‑flow operation and system components designed for 2.1 mm ID columns.
Why Choose Bidentate C18™ 2.o (2.1 × 150 mm)
This format is selected when:
Maximum separation power is required
Shorter columns (50–100 mm) do not provide enough resolution
Highly hydrophobic or non‑polar analytes are difficult to retain
Near‑UHPLC efficiency is desired on a non‑UHPLC system
The combination of 2.2 µm particles and 150 mm length delivers superior resolution while maintaining the durability and method flexibility characteristic of TYPE‑C™ silica hydride phases.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Reduced silanol activity and secondary interactions
Faster equilibration than conventional C18 columns
Extremely robust column lifetime
Bidentate C18™ 2.o Bonding
Two‑point attachment via direct Si–C bonds
Unique selectivity not achievable with standard C18 phases
Excellent retention of highly hydrophobic compounds
Near‑UHPLC Efficiency (2.2 µm)
Significantly increased efficiency vs. 3–5 µm columns
High resolution on standard HPLC systems
Ideal for demanding analytical separations
LC‑MS‑Compatible Geometry
2.1 mm ID enhances sensitivity and reduces solvent use
150 mm length maximizes resolving power
Suitable for LC‑MS workflows with instruments designed for this ID
System Requirements
Because of the small particle size and narrow‑bore, long‑format geometry, optimal performance requires:
A pump capable of precise, stable low‑flow delivery
System components (injector, tubing, detector flow cell) designed for 2.1 mm ID columns
Systems not configured for low‑flow operation may not achieve expected performance.
Typical Applications
High‑resolution analytical HPLC
LC‑MS and LC‑MS/MS with increased resolution requirements
Very hydrophobic and non‑polar compounds
Aqueous‑dominant mobile‑phase methods
Method development for difficult separations