Catalog Number - 40260-05P-2

The 40260‑05P‑2 Diol 2.o™ HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, combining the classical selectivity of Diol chemistry with the performance advantages of a silica hydride surface. The 2.2 µm particle size delivers increased efficiency, excellent selectivity, and faster equilibration than conventional bonded‑silica diol phases, while remaining compatible with non‑UHPLC instruments under typical operating conditions.

This Diol 2.o™ column is considered “near‑UHPLC”, allowing laboratories to achieve sharper peaks and higher efficiency on standard HPLC systems without requiring UHPLC‑rated pressures.

Application Focus: Diol Chemistry for HILIC & Polar Compounds

Diol stationary phases are widely selected for HILIC and normal‑phase chromatography due to their strong hydrophilicity and predictable hydrogen‑bonding interactions. The Diol 2.o™ phase is particularly effective for polar compounds that exhibit poor or no retention in reversed‑phase methods, including:

• Amino acids

• Polar metabolites

• Small biological and biomarker compounds

• Other highly water‑soluble analytes

When operated in HILIC mode, this column is considered mass‑spectrometry friendly, supporting MS‑compatible mobile phases while maintaining strong retention and reproducible selectivity for polar analytes.

Cogent TYPE‑C™ Performance Advantages

Unlike traditional bonded‑silica diol columns, Diol 2.o™ is built on a silica hydride surface, which provides measurable advantages in routine operation:

• Faster equilibration than conventional silica‑based diol phases

• Improved peak efficiency from the smaller 2.2 µm particles

• Excellent selectivity for difficult polar compounds

• Greater stability and reproducibility during gradient HILIC methods

The silica hydride surface helps reduce excessive water‑layer dependence, resulting in more consistent retention and shorter re‑equilibration times between runs.

Why Choose the 2.1 mm × 50 mm Format

The short, narrow‑bore geometry is optimized for speed, sensitivity, and solvent efficiency, making it a favorite for LC‑MS workflows:

• Short run times and rapid throughput

• Fast equilibration between injections

• Reduced solvent consumption and waste

• Lower flow to the MS source, improving ionization efficiency

This size is especially well‑suited for high‑throughput analysis of polar analytes by LC‑MS and LC‑MS/MS.

Particle Size and Efficiency

The 2.2 µm particle size provides near‑UHPLC separation efficiency, producing narrow, symmetrical peaks while avoiding the high backpressures typically associated with sub‑2 µm UHPLC columns under common operating conditions.

LC‑MS‑Focused Operation

The reduced internal volume of the 2.1 mm ID column supports:

• Improved signal‑to‑noise ratios

• Sharper peak shapes

• Reliable quantitative performance

These attributes make the Diol 2.o™ column well suited for metabolomics, bioanalysis, and trace‑level polar compound workflows.

System Requirements and Flow Rates

• Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min

• System capability: Requires a pump capable of accurate, stable low‑flow delivery

• Important note: Not all standard HPLC pumps or flow cells are suitable for precise operation at these reduced flow rates

Final flow‑rate selection should account for mobile‑phase viscosity, column temperature, and system pressure limits.