Catalog Number - 49130-06

The 49130‑06 Caffeine Test Mixture Solution is a ready‑to‑use analytical standard designed for HPLC system suitability testing, detector verification, and method development workflows.

Formulated using caffeine, a well‑characterized compound with strong and reproducible UV absorbance, this solution provides consistent chromatographic behavior for evaluating system and column performance.

Caffeine – Proven Analytical Standard

• Strong, well‑defined UV absorbance characteristics

• Highly reproducible retention behavior in reversed‑phase HPLC

• Widely accepted for system suitability and method validation workflows

Typical Detection Wavelengths

• ~205 nm (low‑UV sensitivity applications)

• ~273 nm (primary caffeine absorbance maximum)

✅ Enables accurate UV detector verification and calibration support

System Suitability & Performance Testing

This solution is commonly used to evaluate:

• Column efficiency

• Peak shape and symmetry

• Retention time reproducibility

• Resolution characteristics

• System repeatability

✅ Essential for routine HPLC performance verification and validation workflows

Formulation & Use

• Supplied as a ready‑to‑use mixture

• Prepared in aqueous / organic solvent system (e.g., water with acetonitrile or methanol) (composition may vary by lot)

• Designed for immediate use without preparation

✅ Reduces setup time and operator variability

Role in Qualification Workflows

• Supports:

  • System suitability testing (SST)

  • Column performance verification

  • Detector calibration support (with wavelength standards)

  • Method development and training

✅ Complements HSQ™ and PQ™ qualification kits and workflows

Workflow Advantages

Reliable Analytical Standard

• Provides consistent chromatographic response

• Suitable for routine verification and validation

Time‑Saving Implementation

• Ready‑to‑use solution eliminates preparation steps

• Ideal for high‑throughput laboratories

Versatile Application

• Used in QC, training, and method development environments

• Supports multiple analytical objectives with a single standard

Typical Applications

• HPLC system suitability testing

• Column performance evaluation

• UV detector verification support

• Analytical method development

• Laboratory training and validation exercises

• Pharmaceutical QC workflows

Important Usage Notes

• ⚠️ This is a made‑to‑order item and cannot be canceled or returned once ordered

• Designed for routine system verification and analytical workflows

• Use according to validated SOPs and system suitability criteria

• Store and handle per recommended conditions for optimal stability

Related Technical Notes

• Spectrum of Caffeine in Resolution Test Mixture (RTM) provides UV absorbance data and chromatographic behavior of caffeine in the RTM.

• Application Note - discusses caffeine quantification in beverages using HPLC, which may use similar standards for calibration.

*This item can be made to order and may have a lead-time of 10-15 business days.