HPLC Column Set, PEEK with a 7.5 mm ID. Includes, a 50 mm x 7.5 mm black, column body tube (5/8-18 threads), plus 2 PEEK 5 um frits, 2 frit spacers & 2 end fittings. ARE-Applied Research brand. 1 EA.

Additional Info:

The 49333‑50 All‑PEEK HPLC Column Set is designed for professional column packers and preparative chromatographers who require bioinert, higher‑capacity column hardware for semi‑preparative separations. The 7.5 mm ID format increases sample loading capacity and throughput, while maintaining a completely metal‑free flow path.

PEEK (polyether ether ketone) column hardware eliminates interactions with stainless steel surfaces, making it ideal for proteins, peptides, nucleic acids, ions, and metal‑sensitive small molecules, where adsorption or catalytic effects can reduce recovery and affect chromatographic accuracy.

✅ Why Choose All‑PEEK Column Hardware

  • True Bioinert Flow Path Prevents analyte interaction with metal surfaces

  • Higher Sample Loading Capacity (7.5 mm ID) Supports semi‑prep and scale‑up workflows

  • Improved Recovery & Peak Integrity Minimizes adsorption and secondary interactions

  • Pack Any Stationary Phase Complete flexibility for custom column development

  • Reusable & Repackable Design Ideal for method development and process scale‑up


Performance & Design

The column body is produced using a proprietary extrusion process that creates a smooth, mirror‑like internal surface, enabling:

  • Uniform and reproducible packing

  • Reduced flow disruption

  • Improved chromatographic efficiency

Constructed from graphite-filled PEEK, the graphite is incorporated before extrusion, not applied as a coating.

Key advantages:

  • No chemical coatings or surface treatments

  • Consistent internal surface chemistry

  • Durable black exterior for long-term use


Included Components

  • 50 mm × 7.5 mm ID All‑PEEK column body (5/8‑18 threads)

  • 2 × 5 µm PEEK frits

  • 2 × frit spacers

  • 2 × PEEK end fittings


Use Case Positioning

7.5 mm ID / 50 mm Length

  • Balanced throughput and separation performance

  • Ideal for semi‑preparative method development and optimization

  • Increased resolution compared to shorter (30 mm) columns


Typical Applications

  • Semi‑Preparative Protein & Peptide Purification

  • Nucleic Acid Analysis and Isolation

  • Ion Chromatography of sensitive compounds

  • Analytical-to-preparative method scale‑up

  • Custom column packing workflows

  • Bioinert LC‑MS applications


⚠️ When to Use PEEK Column Hardware

Select All‑PEEK columns when:

  • Analytes are sensitive to metal interaction

  • Higher sample loading capacity is required

  • Working with biomolecules or coordination-sensitive compounds


Packing & Compatibility

For optimal performance:

  • Use with ARE‑Applied Research™ All‑PEEK slurry reservoir kits

  • Select frit porosity appropriate for particle size and column scale

  • Maintain a fully bioinert packing environment

Read guidelines for packing All‑PEEK HPLC columns and achieving consistent column performance


Specifications

  • Hardware Type: All‑PEEK Column Set

  • Internal Diameter: 7.5 mm

  • Length: 50 mm

  • Thread Type: 5/8‑18

  • Material: Extruded PEEK (graphite‑filled)

  • Frits: 5 µm PEEK

  • Additional Components: Frit spacers included

  • Flow Path: Metal‑free (bioinert)

  • Surface Treatment: None (no coatings)

  • Reusability: Repackable

  • Brand: ARE‑Applied Research™

  • Unit: 1 Each

Resources & Ordering Options

 

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