Catalog Number - 49335-03

The 49335‑03 All‑PEEK HPLC Column Set is designed for professional column packers and chromatographers who require complete control over bioinert column construction in a fast, short-column analytical format. This 4.6 mm ID hardware set enables in‑house packing of custom HPLC columns using virtually any stationary phase.

Constructed entirely from PEEK (polyether ether ketone), this column provides a fully metal‑free flow path, helping prevent undesirable interactions between analytes and stainless steel surfaces. This is especially important for proteins, peptides, nucleic acids, ions, and metal‑sensitive small molecules, where adsorption or catalytic effects can compromise recovery and data quality.

✅ Why Choose All‑PEEK Column Hardware

• True Bioinert Flow Path Eliminates metal contact to protect sensitive analytes

• Improved Recovery & Peak Integrity Reduces adsorption and secondary interactions

• Pack Any Stationary Phase Full flexibility for method development and custom separations

• Reusable & Repackable Design Ideal for R&D and screening workflows

• Standard 4.6 mm Format (1/2‑20 Threads) Compatible with analytical HPLC packing systems

Performance & Design

The column body is manufactured from extruded PEEK tubing using a proprietary process that produces a smooth, mirror‑like internal surface, helping to:

• Improve packing consistency

• Reduce flow disruption

• Enhance chromatographic efficiency

Unlike coated or chemically modified systems, this column is:

• Not surface treated

• Not chemically modified

• Inherently bioinert by material design

A controlled amount of graphite is incorporated into the PEEK prior to extrusion, producing the black finish while maintaining durability and structural stability.

Included Components

• 1 × 30 mm × 4.6 mm ID PEEK Column Body (1/2‑20 threads)

• 2 × 2 µm PEEK Frits

• 2 × PEEK End Fittings

✔ Complete hardware set for packing one column

Use Case Positioning

4.6 mm ID / 30 mm Length

• Ultra-short column format → Maximum speed and rapid separations

• Analytical scale → Compatible with standard HPLC systems

• Low backpressure → Fast screening and method scouting

Typical Applications

• Rapid method development and screening

• Protein & peptide analysis

• Nucleic acid separations

• Ion chromatography & metal‑sensitive compounds

• Bioanalytical workflows

• Stationary phase evaluation and scouting

⚠️ When to Use PEEK Column Hardware

Choose All‑PEEK columns when:

• Samples may adsorb to or react with metal surfaces

• Recovery and reproducibility are critical

• Working with biomolecules or coordination-sensitive analytes

• A fully bioinert LC system is required

Packing & Compatibility

For optimal results:

• Use with All‑PEEK Slurry Reservoir Kits sized for short 4.6 mm columns

• Select frit porosity appropriate to particle size

• Maintain a fully bioinert packing environment

Specifications

• Hardware Type: All‑PEEK Column Set

• Internal Diameter: 4.6 mm

• Length: 30 mm

• Thread Type: 1/2‑20

• Material: Extruded PEEK (graphite‑filled)

• Frits: 2 µm PEEK

• Flow Path: Metal‑free (bioinert)

• Surface Treatment: None

• Reusability: Repackable

• Brand: ARE‑Applied Research™

• Unit: 1 Each

Resources & Ordering Options

• View All‑PEEK Slurry Reservoir Kits for 4.6 mm Column Packing

• Read Guidelines for Packing All‑PEEK HPLC Columns and Achieving Consistent Performance