HPLC Column Set, PEEK with a 4.6 mm ID. Includes, a 250 mm x 4.6 mm black, column body tube (1/2-20 threads), plus 2 PEEK 2 um frits & 2 end fittings. ARE-Applied Research brand. 1 EA.
Additional Info:
The 49335‑250 All‑PEEK HPLC Column Set is designed for professional column packers and advanced chromatographers who require maximum separation efficiency and full control over bioinert column construction. The 250 mm length configuration delivers extended retention, higher resolution, and greater peak capacity, making it ideal for complex and highly demanding separations.
PEEK (polyether ether ketone) column hardware provides a completely metal‑free flow path, eliminating unwanted analyte interaction with stainless steel. This is critical for proteins, peptides, nucleic acids, ions, and metal‑sensitive small molecules, where adsorption or catalytic effects can reduce recovery and distort peak shape.
✅ Why Choose All‑PEEK Column Hardware
True Bioinert Flow Path Eliminates interaction with metal surfaces
Maximum Recovery & Peak Integrity Reduces adsorption and improves reproducibility
Pack Any Stationary Phase Complete flexibility for custom column development
Reusable & Repackable Design Ideal for advanced method development and validation
Standard 1/2‑20 Thread Format Compatible with common 4.6 mm column hardware systems
Performance & Design
The column body is manufactured using a proprietary extrusion process that produces a smooth, mirror‑like internal surface, supporting:
Highly uniform and reproducible packing
Reduced turbulence in the flow path
Improved chromatographic efficiency
This column is constructed from graphite-filled PEEK, where graphite is incorporated prior to extrusion, not applied as a coating.
Key benefits:
No chemical surface treatments
Consistent internal chemistry across batches
Durable black outer finish for long-term use
Included Components
250 mm × 4.6 mm ID All‑PEEK column body (1/2‑20 threads)
2 × 2 µm PEEK frits
2 × PEEK end fittings
Use Case Positioning
4.6 mm ID / 250 mm Length
Maximum resolution and peak capacity
Ideal for complex mixtures and difficult separations
Best suited for method validation, impurity profiling, and regulatory workflows
Typical Applications
Protein & Peptide Analysis (high-resolution separations)
Nucleic Acid Characterization
Ion Chromatography
Impurity Profiling & Method Validation
Advanced Bioanalytical Workflows
LC‑MS requiring metal‑free fluid paths
⚠️ When to Use PEEK Column Hardware
Choose All‑PEEK columns when:
Analytes are sensitive to metal interaction
Maximum recovery, selectivity, and reproducibility are required
Working with biomolecules or metal-coordinating compounds
Packing & Compatibility
For optimal performance:
Use with ARE‑Applied Research™ All‑PEEK slurry reservoir kits
Select frit porosity appropriate for particle size
Maintain a fully bioinert packing and operating environment
Read guidelines for packing All‑PEEK HPLC columns and achieving consistent column performance
Specifications
Hardware Type: All‑PEEK Column Set
Internal Diameter: 4.6 mm
Length: 250 mm
Thread Type: 1/2‑20
Material: Extruded PEEK (graphite‑filled)
Frits: 2 µm PEEK
Flow Path: Metal‑free (bioinert)
Surface Treatment: None (no coatings)
Reusability: Repackable
Brand: ARE‑Applied Research™
Unit: 1 Each
Resources & Ordering Options