Catalog Number - 59051-15P

MicroBee Cyano™ is a traditional cyanopropyl HPLC stationary phase developed for laboratories requiring an economical alternative to legacy cyano columns used in established analytical methods. As part of the Cogent™ Similars product line, it has been trusted by chromatographers for many years when cyano selectivity is preferred over conventional C18 phases. The bonded cyano functionality provides moderate polarity and unique retention characteristics that can improve selectivity for compounds that are difficult to separate with traditional reversed-phase columns.

59051-15P is a 150 mm x 4.6 mm ID analytical column packed with 10 µm, 125Å MicroBee Cyano particles. The 150 mm length provides an effective balance of chromatographic resolution and run time, making it suitable for routine analytical methods, quality control testing, and method transfer applications. The industry-standard 4.6 mm ID format typically performs optimally at flow rates of approximately 1.0–2.0 mL/min on conventional HPLC systems.

This USP L10 column is manufactured using a non-endcapped cyanopropyl bonded phase attached to Type-A silica. It serves as an excellent alternative for methods developed on Microbondapak™ Cyano and similar cyano columns. The phase is commonly used for polar and moderately hydrophobic compounds and can provide selectivity that differs significantly from traditional C18 stationary phases, making it a useful option during method development and optimization.

Key Benefits

• Traditional cyanopropyl stationary phase

• Suitable alternative for USP L10 methods

• Unique selectivity compared to C18 columns

• Non-endcapped cyano bonded phase

• 125Å pore size for broad analyte compatibility

• Cost-effective Cogent Similars column

• Suitable for polar and moderately hydrophobic compounds

• Compatible with legacy cyano methods

Typical Applications

• USP L10 methods

• Reversed-phase HPLC

• Method development

• Pharmaceutical analysis

• Quality control testing

• Alternative selectivity screening

• Polar compound separations

• Legacy cyano column replacement

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee Cyano

• USP Classification: L10

• Particle Size: 10 µm

• Particle Type: Type-A Silica

• Bonded Phase: Cyanopropyl

• Endcapped: No

• Pore Size: 125Å

• Column Dimensions: 4.6 mm ID x 150 mm

• Length: 150 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• USP L10 column classification

• Non-endcapped cyanopropyl stationary phase

• Compatible with conventional HPLC systems

• Industry-standard 4.6 mm ID analytical format

• Ideal replacement for many legacy cyano methods

• Alternative selectivity to traditional C18 columns

• Suitable for routine analytical HPLC testing

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