Catalog Number - 59300-03

MicroBee Cyano™ is a traditional cyanopropyl HPLC stationary phase developed for laboratories requiring an economical alternative to legacy cyano columns used in established analytical methods. As part of the Cogent™ Similars product line, it has been widely used for many years when cyano selectivity is preferred over conventional C18 phases. The bonded cyano functionality provides moderate polarity and unique retention characteristics that can improve selectivity for compounds that are difficult to separate using traditional reversed-phase columns.

59300-03 is a 300 mm x 3.9 mm ID analytical column packed with 10 µm, 125Å MicroBee Cyano particles. This dimension closely matches the format used in many historical HPLC methods developed on Microbondapak™ and similar cyano columns, making it particularly useful for method transfer and replacement applications. The 300 mm length provides maximum resolving power and increased theoretical plate counts for challenging separations where chromatographic resolution is more important than analysis speed. The 3.9 mm ID format typically performs well at flow rates of approximately 0.8–1.5 mL/min on conventional HPLC systems.

This USP L10 column is manufactured using a non-endcapped cyanopropyl bonded phase attached to Type-A silica. It serves as an excellent alternative for methods developed on Microbondapak™ Cyano and similar cyano columns. The phase is commonly used for polar and moderately hydrophobic compounds and offers selectivity distinct from traditional C18 stationary phases, making it valuable for method development, validation, and difficult separations requiring alternative retention mechanisms. As one of the most popular MicroBee Cyano column configurations, it remains a preferred choice for laboratories seeking dependable cyano selectivity in a legacy column format.

Key Benefits

• Traditional cyanopropyl stationary phase

• Suitable alternative for USP L10 methods

• Compatible with many legacy Microbondapak™ methods

• Maximum resolution from 300 mm column length

• Unique selectivity compared to C18 columns

• Non-endcapped cyano bonded phase

• 125Å pore size for broad analyte compatibility

• One of the most popular MicroBee Cyano columns

Typical Applications

• USP L10 methods

• Reversed-phase HPLC

• Method development

• Pharmaceutical analysis

• Impurity profiling

• Quality control testing

• Polar compound separations

• Legacy cyano column replacement

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee Cyano

• USP Classification: L10

• Particle Size: 10 µm

• Particle Type: Type-A Silica

• Bonded Phase: Cyanopropyl

• Endcapped: No

• Pore Size: 125Å

• Column Dimensions: 3.9 mm ID x 300 mm

• Length: 300 mm

• Recommended Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Important Notes

• One of the most popular MicroBee Cyano column configurations

• USP L10 column classification

• Non-endcapped cyanopropyl stationary phase

• Designed for reversed-phase chromatography

• Ideal for legacy method replacement

• Compatible with conventional HPLC systems

• Extended column length provides maximum resolving power

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