Catalog Number - 62318-05P

Hyper Similars C18™ is a traditional reversed-phase HPLC stationary phase designed as a high-quality alternative to established C18 methods. Part of the Cogent™ Similars product line, it is manufactured on high-purity Type-B spherical silica with a fully endcapped octadecyl (C18) bonded phase. Its silica surface characteristics closely resemble those of legacy Hypersil® and Spherisorb® columns, making it an excellent choice for USP L1 methods, method transfer projects, and replacement of discontinued C18 columns while maintaining familiar retention, selectivity, and reproducibility.

62318-05P is a 50 mm x 4.6 mm ID analytical column packed with 3 µm, 150Å Hyper Similars C18 particles. The short 50 mm length is optimized for fast separations, reduced solvent consumption, and high sample throughput. The 3 µm particle size provides greater efficiency and sharper peaks than comparable 5 µm packings, although higher operating backpressure should be expected. The industry-standard 4.6 mm ID format typically operates at approximately 1.0–2.0 mL/min and is well suited for rapid reversed-phase methods on conventional HPLC systems.

This USP L1 column is ideal for hydrophobic and neutral compounds requiring strong reversed-phase retention. Common applications include pharmaceutical analysis, long-chain fatty acid separations, quality control testing, method development, and routine analytical HPLC where fast run times are desired.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L1 methods

• Comparable selectivity to Hypersil® and Spherisorb® C18 columns

• Fast analysis with 50 mm column length

• Higher efficiency with 3 µm particles

• Sharper peaks and improved resolution

• Excellent for hydrophobic and neutral compounds

• End-capped C18 bonded phase

Typical Applications

• USP L1 methods

• Reversed-phase HPLC

• Pharmaceutical analysis

• Quality control testing

• Method development

• Long-chain fatty acid analysis

• Fast analytical separations

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Hyper Similars C18

• USP Classification: L1

• Particle Size: 3 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C18 (Octadecyl)

• Endcapped: Yes

• Pore Size: 150Å

• Column Dimensions: 4.6 mm ID x 50 mm

• Length: 50 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Short column length provides rapid analyses

• 3 µm particles provide higher efficiency and sharper peaks

• Higher backpressure than comparable 5 µm columns

• Compatible with conventional HPLC systems

• Excellent alternative for many USP L1 methods

• Ideal for high-throughput workflows

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