Catalog Number - 62318-05P-2

Hyper Similars C18™ is a traditional reversed-phase HPLC stationary phase designed as a high-quality alternative to established C18 methods. Part of the Cogent™ Similars product line, it is manufactured on high-purity Type-B spherical silica with a fully endcapped octadecyl (C18) bonded phase. The silica surface characteristics closely resemble those of legacy Hypersil® and Spherisorb® columns, making it an excellent choice for USP L1 methods, method transfer projects, and replacement of discontinued C18 columns while maintaining familiar retention, selectivity, and reproducibility.

62318-05P-2 is a 50 mm x 2.1 mm ID narrow-bore analytical column packed with 3 µm, 150Å Hyper Similars C18 particles. The short 50 mm length is optimized for fast analyses, reduced solvent consumption, and high sample throughput. The 3 µm particle size provides greater efficiency and sharper peaks than comparable 5 µm columns, although higher operating backpressure should be expected. This narrow-bore format is particularly well suited for LC-MS applications and typically operates at approximately 0.2–0.3 mL/min. Reliable performance requires a system capable of precise low-flow delivery and low-volume flow path design.

This USP L1 column is ideal for hydrophobic and neutral compounds analyzed by reversed-phase HPLC and LC-MS. The combination of strong C18 retention, fast run times, and low solvent consumption makes it a popular choice for pharmaceutical analysis, long-chain fatty acids, bioanalytical methods, and high-sensitivity LC-MS workflows.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L1 methods

• Comparable selectivity to Hypersil® and Spherisorb® C18 columns

• Narrow-bore format ideal for LC-MS

• Fast analysis with 50 mm column length

• Higher efficiency with 3 µm particles

• Reduced solvent consumption

• End-capped C18 bonded phase

Typical Applications

• USP L1 methods

• LC-MS analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Bioanalytical methods

• Long-chain fatty acid analysis

• Fast analytical separations

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Hyper Similars C18

• USP Classification: L1

• Particle Size: 3 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C18 (Octadecyl)

• Endcapped: Yes

• Pore Size: 150Å

• Column Dimensions: 2.1 mm ID x 50 mm

• Length: 50 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Narrow-bore format optimized for LC-MS applications

• Requires precise low-flow pumping capability

• 3 µm particles provide higher efficiency and sharper peaks

• Higher backpressure than comparable 5 µm columns

• Reduced solvent consumption compared to 4.6 mm ID columns

• Excellent for high-sensitivity analyses

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