Catalog Number - 62318-10P-2

Hyper Similars C18™ is a traditional reversed-phase HPLC stationary phase designed as a high-quality alternative to established C18 methods. Part of the Cogent™ Similars product line, it is manufactured on high-purity Type-B spherical silica with a fully endcapped octadecyl (C18) bonded phase. The silica surface characteristics closely resemble those of legacy Hypersil® and Spherisorb® columns, making it an excellent choice for USP L1 methods, method transfer projects, and replacement of discontinued C18 columns. This stationary phase delivers the strong hydrophobic retention, reproducibility, and broad applicability expected from a conventional C18 chemistry.

62318-10P-2 is a 100 mm x 2.1 mm ID narrow-bore analytical column packed with 3 µm, 150Å Hyper Similars C18 particles. The 100 mm length provides a balance of chromatographic resolution and analytical speed, while the 3 µm particle size produces higher efficiency and sharper peaks than comparable 5 µm columns. The 2.1 mm ID format significantly reduces solvent consumption and is optimized for LC-MS applications. Typical flow rates are approximately 0.2–0.3 mL/min, and reliable operation requires an HPLC or LC-MS system capable of precise low-flow delivery with appropriately sized flow cells and plumbing.

This USP L1 column is ideal for hydrophobic and neutral compounds requiring strong reversed-phase retention and enhanced MS compatibility. It is frequently selected for pharmaceutical analysis, bioanalytical methods, long-chain fatty acid separations, and LC-MS workflows where improved sensitivity, reduced solvent usage, and efficient separations are important.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L1 methods

• Comparable selectivity to Hypersil® and Spherisorb® C18 columns

• Narrow-bore format ideal for LC-MS applications

• Balanced speed and resolution with 100 mm length

• Higher efficiency with 3 µm particles

• Reduced solvent consumption

• End-capped C18 bonded phase

Typical Applications

• USP L1 methods

• LC-MS analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Bioanalytical methods

• Long-chain fatty acid analysis

• Method development

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Hyper Similars C18

• USP Classification: L1

• Particle Size: 3 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C18 (Octadecyl)

• Endcapped: Yes

• Pore Size: 150Å

• Column Dimensions: 2.1 mm ID x 100 mm

• Length: 100 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Narrow-bore format optimized for LC-MS applications

• Requires precise low-flow pumping capability

• 3 µm particles provide higher efficiency and sharper peaks

• Higher backpressure than comparable 5 µm columns

• Reduced solvent consumption compared to 4.6 mm ID columns

• Excellent for high-sensitivity analyses

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