Catalog Number - 62318-25P

Hyper Similars C18™ is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped octadecyl (C18) bonded phase. Designed as a cost-effective alternative to legacy Hypersil® and Spherisorb® columns, it offers comparable chromatographic behavior for USP L1 methods while providing the strong hydrophobic retention, reproducibility, and broad applicability expected from a conventional C18 stationary phase.

62318-25P is a 250 mm x 4.6 mm ID analytical column packed with 3 µm, 150Å Hyper Similars C18 particles. The extended 250 mm length provides increased theoretical plate counts and enhanced resolving power for impurity profiling, complex sample matrices, and challenging separations where maximum resolution is required. The 3 µm particle size delivers higher efficiency and sharper peak shapes than comparable 5 µm packings, although higher operating backpressure should be expected. Typical operating flow rates are approximately 1.0–2.0 mL/min on conventional HPLC systems.

This USP L1 column is ideal for hydrophobic and neutral compounds requiring strong reversed-phase retention and high chromatographic efficiency. Common applications include pharmaceutical analysis, long-chain fatty acid separations, impurity profiling, method development, and quality control testing where additional separation is desired.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L1 methods

• Comparable selectivity to Hypersil® and Spherisorb® C18 columns

• Increased resolution from 250 mm column length

• Higher efficiency with 3 µm particles

• Sharper peaks and improved separation performance

• Excellent for hydrophobic and neutral compounds

• End-capped C18 bonded phase

Typical Applications

• USP L1 methods

• Reversed-phase HPLC

• Pharmaceutical analysis

• Impurity profiling

• Method development

• Long-chain fatty acid analysis

• Quality control testing

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Hyper Similars C18

• USP Classification: L1

• Particle Size: 3 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C18 (Octadecyl)

• Endcapped: Yes

• Pore Size: 150Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Longer column length provides increased resolving power

• 3 µm particles provide higher efficiency and sharper peaks

• Higher backpressure than comparable 5 µm columns

• Compatible with conventional HPLC systems

• Excellent alternative for many USP L1 methods

• Ideal for complex separations and impurity analysis

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