HPLC Column, Hyper Similars C18, 3um, 4.0mm ID x 250mm Length, 150A. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Hyper Similars C18™ is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped octadecyl (C18) bonded phase. Designed as a cost-effective alternative to legacy Hypersil® and Spherisorb® columns, it offers comparable chromatographic behavior for USP L1 methods while providing the strong hydrophobic retention, reproducibility, and broad applicability expected from a conventional C18 stationary phase.
62318-25P-4 is a 250 mm x 4.0 mm ID analytical column packed with 3 µm, 150Å Hyper Similars C18 particles. The extended 250 mm length provides increased theoretical plate counts and enhanced resolving power for impurity profiling, complex sample matrices, and challenging separations where maximum resolution is required. The 3 µm particle size delivers higher efficiency and sharper peak shapes than comparable 5 µm packings, although higher operating backpressure should be expected. Typical operating flow rates are approximately 0.8–1.5 mL/min on conventional HPLC systems, making this configuration useful for reproducing legacy 4.0 mm ID methods.
This USP L1 column is ideal for hydrophobic and neutral compounds requiring strong reversed-phase retention and high chromatographic efficiency. Common applications include pharmaceutical analysis, long-chain fatty acid separations, impurity profiling, method development, and quality control testing. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
Type-B spherical silica stationary phase
Suitable alternative for USP L1 methods
Comparable selectivity to Hypersil® and Spherisorb® C18 columns
Increased resolution from 250 mm column length
Higher efficiency with 3 µm particles
Sharper peaks and improved separation performance
Excellent for hydrophobic and neutral compounds
End-capped C18 bonded phase
Typical Applications
USP L1 methods
Reversed-phase HPLC
Pharmaceutical analysis
Impurity profiling
Method development
Long-chain fatty acid analysis
Quality control testing
Method transfer and validation
Specifications
Product Type: HPLC Column
Stationary Phase: Hyper Similars C18
USP Classification: L1
Particle Size: 3 µm
Particle Type: Type-B Spherical Silica
Bonded Phase: C18 (Octadecyl)
Endcapped: Yes
Pore Size: 150Å
Column Dimensions: 4.0 mm ID x 250 mm
Length: 250 mm
Recommended Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Special Order Item Information
This item is a Special Order Item manufactured specifically to customer requirements.
Special Order Items cannot be canceled or returned once an order has been placed.
Manufactured upon receipt of order.
May have extended lead times compared to stocked products.
Important Notes
Designed for reversed-phase chromatography
Longer column length provides increased resolving power
3 µm particles provide higher efficiency and sharper peaks
Higher backpressure than comparable 5 µm columns
Compatible with conventional HPLC systems
Excellent alternative for many USP L1 methods
Special Order Item