Catalog Number - 62518-05P-2

Hyper Similars C18™ is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped octadecyl (C18) bonded phase. Designed as a cost-effective alternative to legacy Hypersil® and Spherisorb® columns, it offers comparable chromatographic behavior for USP L1 methods while providing the strong hydrophobic retention, reproducibility, and broad applicability expected from a conventional C18 stationary phase.

62518-05P-2 is a 50 mm x 2.1 mm ID narrow-bore analytical column packed with 5 µm, 150Å Hyper Similars C18 particles. The short 50 mm length is optimized for fast analyses and reduced solvent consumption, while the 2.1 mm ID format is particularly well suited for LC-MS applications. Typical operating flow rates are approximately 0.2–0.3 mL/min and require a system capable of precise low-flow delivery with low-volume flow cells and plumbing. The 5 µm particle size provides reliable performance with moderate operating pressure.

This USP L1 column is ideal for hydrophobic and neutral compounds requiring strong reversed-phase retention. Its narrow-bore format, low-flow operation, and fast run times make it a popular choice for LC-MS methods, pharmaceutical analysis, long-chain fatty acid separations, and high-sensitivity analytical workflows.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L1 methods

• Comparable selectivity to Hypersil® and Spherisorb® C18 columns

• Narrow-bore format ideal for LC-MS applications

• Fast analysis with 50 mm column length

• Strong hydrophobic retention

• Reduced solvent consumption

• End-capped C18 bonded phase

Typical Applications

• USP L1 methods

• LC-MS analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Long-chain fatty acid analysis

• Quality control testing

• Fast analytical separations

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Hyper Similars C18

• USP Classification: L1

• Particle Size: 5 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C18 (Octadecyl)

• Endcapped: Yes

• Pore Size: 150Å

• Column Dimensions: 2.1 mm ID x 50 mm

• Length: 50 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Narrow-bore format optimized for LC-MS applications

• Requires an HPLC or LC-MS system capable of reliable low-flow operation

• Lower operating pressure than comparable 3 µm columns

• Reduced solvent consumption compared to 4.6 mm ID columns

• Excellent alternative for many USP L1 methods

• Ideal for high-throughput LC-MS workflows

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