HPLC Column, Nova Similars C18, 4um, 2.1mm ID x 50mm Length. Cogent Brand. 1 EA.

Additional Information:

Nova Similars C18™ is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped octadecyl (C18) bonded phase. Designed as a cost-effective alternative to Waters™ Nova-Pak® columns, it offers comparable chromatographic behavior for USP L1 methods while providing the strong hydrophobic retention, reproducibility, and broad applicability expected from a conventional C18 stationary phase.

64418-05P-2 is a 50 mm x 2.1 mm ID narrow-bore analytical column packed with 4 µm Nova Similars C18 particles. The short 50 mm length is optimized for fast analyses and reduced solvent consumption, while the 2.1 mm ID format is especially well suited for LC-MS applications. The 4 µm particle size provides improved efficiency and somewhat sharper peaks than comparable 5 µm columns while maintaining moderate operating pressure. Typical flow rates are approximately 0.2–0.3 mL/min and require a system capable of precise low-flow delivery with low-volume flow cells and plumbing.

This USP L1 column is ideal for hydrophobic and neutral compounds requiring strong reversed-phase retention. Its narrow-bore format, low-flow operation, and rapid analysis capability make it a popular choice for LC-MS methods, pharmaceutical analysis, long-chain fatty acid separations, and high-sensitivity analytical workflows.

Key Benefits

  • Type-B spherical silica stationary phase

  • Suitable alternative for USP L1 methods

  • Comparable selectivity to Waters™ Nova-Pak® C18 columns

  • Narrow-bore format ideal for LC-MS applications

  • Fast analysis with 50 mm column length

  • Higher efficiency than comparable 5 µm columns

  • Reduced solvent consumption

  • End-capped C18 bonded phase

Typical Applications

  • USP L1 methods

  • LC-MS analysis

  • Reversed-phase HPLC

  • Pharmaceutical analysis

  • Long-chain fatty acid analysis

  • Quality control testing

  • Fast analytical separations

  • Method transfer and validation

Specifications

  • Product Type: HPLC Column

  • Stationary Phase: Nova Similars C18

  • USP Classification: L1

  • Particle Size: 4 µm

  • Particle Type: Type-B Spherical Silica

  • Bonded Phase: C18 (Octadecyl)

  • Endcapped: Yes

  • Column Dimensions: 2.1 mm ID x 50 mm

  • Length: 50 mm

  • Recommended Flow Rate: 0.2–0.3 mL/min

  • Brand: Cogent

Important Notes

  • Designed for reversed-phase chromatography

  • Narrow-bore format optimized for LC-MS applications

  • Requires an HPLC or LC-MS system capable of reliable low-flow operation

  • Higher efficiency than comparable 5 µm columns

  • Reduced solvent consumption compared to 4.6 mm ID columns

  • Excellent alternative for many USP L1 methods

  • Ideal for high-throughput LC-MS workflows

 

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