Catalog Number - 64424-05P-2

Nova Similars Phenyl™ is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped phenyl bonded phase. The stationary phase consists of a four-carbon linker terminating in a phenyl functional group, providing unique selectivity through a combination of hydrophobic and π-π interactions. Designed as a cost-effective alternative to Waters™ Nova-Pak® Phenyl columns, it offers comparable chromatographic behavior while maintaining the reliability, reproducibility, and performance required for demanding analytical methods.

64424-05P-2 is a 50 mm x 2.1 mm ID narrow-bore analytical column packed with 4 µm Nova Similars Phenyl particles. The short 50 mm length is optimized for rapid analyses and reduced solvent consumption, while the 2.1 mm ID format is particularly well suited for LC-MS applications. The 4 µm particle size provides improved efficiency and sharper peaks than comparable 5 µm columns, while maintaining moderate operating pressure. Typical operating flow rates are approximately 0.2–0.3 mL/min and require a system capable of precise low-flow delivery with low-volume plumbing and flow cells.

This column is particularly useful for aromatic compounds where phenyl selectivity can provide different retention behavior than traditional C18 phases. Common applications include pharmaceutical analysis, aromatic hydrocarbons, metabolite studies, environmental testing, and LC-MS methods requiring enhanced aromatic selectivity and sensitivity.

Key Benefits

• Type-B spherical silica stationary phase

• Fully endcapped phenyl bonded phase

• Enhanced aromatic compound selectivity

• Unique π-π interaction mechanism

• Narrow-bore format ideal for LC-MS applications

• Fast analysis with 50 mm column length

• Higher efficiency than comparable 5 µm columns

• Reduced solvent consumption

Typical Applications

• LC-MS analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Aromatic compound separations

• Environmental testing

• Metabolite analysis

• Method development

• Quality control testing

Specifications

• Product Type: HPLC Column

• Stationary Phase: Nova Similars Phenyl

• USP Classification: L11

• Particle Size: 4 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: Phenyl

• Endcapped: Fully

• Column Dimensions: 2.1 mm ID x 50 mm

• Length: 50 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Particularly useful for aromatic compounds

• Narrow-bore format optimized for LC-MS applications

• Requires an HPLC or LC-MS system capable of reliable low-flow operation

• Higher efficiency than comparable 5 µm columns

• Reduced solvent consumption compared to 4.6 mm ID columns

• Provides unique selectivity compared to traditional C18 phases

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