Catalog Number - 67090-25P

Cogent IEX WAX is a weak anion exchange (WAX) HPLC stationary phase manufactured using high-purity silica and diethylaminopropyl functional groups that provide pH-dependent positive charge characteristics. This weak anion exchange mechanism offers selective retention and separation of negatively charged analytes while allowing greater control of retention through mobile phase pH adjustments. The stationary phase is particularly useful for organic acids, biomolecules, and other anionic compounds and serves as an excellent alternative for USP L9 methods requiring weak anion exchange selectivity.

67090-25P is a 250 mm x 4.6 mm ID analytical column packed with 10 µm WAX particles with a 100 Å pore size. The extended 250 mm length provides increased theoretical plate counts and maximum resolving power for complex ion exchange separations, impurity profiling, purification studies, and challenging analytical applications requiring enhanced separation of closely related anionic compounds. The 10 µm particle size provides low backpressure and supports higher flow rates than smaller particle packings. Typical operating flow rates are approximately 1.0–3.0 mL/min on conventional HPLC systems.

This USP L9 column is particularly useful for the separation and purification of negatively charged compounds, including organic acids, acidic pharmaceuticals, nucleotides, and other anionic species. The weak anion exchange functionality allows chromatographers to optimize selectivity through pH control while maintaining excellent efficiency and reproducibility. This is one of the most popular Cogent ion exchange column configurations for demanding WAX separations requiring maximum chromatographic resolution.

Key Benefits

• Weak anion exchange (WAX) stationary phase

• Diethylaminopropyl functional groups

• pH-dependent retention characteristics

• Suitable alternative for USP L9 methods

• 10 µm particles for lower backpressure

• Supports higher flow rates

• Maximum resolving power from 250 mm column length

• High-purity silica support

Typical Applications

• USP L9 methods

• Ion exchange chromatography

• Organic acid analysis

• Acidic pharmaceutical compounds

• Biomolecule analysis

• Impurity profiling

• Purification studies

• Quality control testing

Specifications

• Product Type: HPLC Column

• Stationary Phase: IEX WAX

• USP Classification: L9

• Chromatography Mode: Weak Anion Exchange

• Functional Group: Diethylaminopropyl

• Functional pH Range: Approximately pH 5–8

• Particle Size: 10 µm

• Pore Size: 100 Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Recommended Flow Rate: 1.0–3.0 mL/min

• Brand: Cogent

Important Notes

• Designed for ion exchange chromatography

• Retains negatively charged analytes

• Retention can be adjusted through mobile phase pH control

• Longer column length provides maximum chromatographic resolution

• Lower backpressure compared to smaller particle columns

• Supports higher flow rates for routine and preparative methods

• Excellent alternative for many USP L9 methods

• One of the most popular Cogent WAX column configurations

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