Catalog Number - 67585-05P-2

Cogent IEX SAX is a strong anion exchange (SAX) HPLC stationary phase manufactured using high-purity silica and quaternary amine functional groups that maintain a permanent positive charge across a wide pH range. This strong anion exchange mechanism provides reliable retention and separation of negatively charged analytes, making the column suitable for ion exchange chromatography methods involving inorganic anions, organic acids, biomolecules, and other ionic compounds. The stationary phase is an excellent alternative for USP L9 methods and applications requiring robust anion exchange selectivity.

67585-05P-2 is a 50 mm x 2.1 mm ID narrow-bore analytical column packed with 5 µm SAX particles with a 100 Å pore size. The short 50 mm length is optimized for fast ion exchange separations and rapid equilibration, while the 2.1 mm ID format minimizes solvent consumption and is well suited for LC-MS compatible ion exchange workflows where low flow rates are desired. The 5 µm particle size provides improved chromatographic efficiency and higher plate counts than larger particle packings. Typical operating flow rates are approximately 0.2–0.3 mL/min and require an HPLC or LC-MS system capable of reliable low-flow operation with low-volume plumbing and flow cells.

This USP L9 column is particularly useful for the separation and purification of negatively charged compounds, including organic acids, inorganic anions, nucleotides, and other ionic species. The combination of strong anion exchange functionality, narrow-bore design, and high-efficiency 5 µm particles makes it ideal for high-sensitivity analytical methods, method development, and demanding ion exchange applications requiring enhanced chromatographic performance.

Key Benefits

• Strong anion exchange (SAX) stationary phase

• Quaternary amine functional groups

• Permanent positive surface charge

• Suitable alternative for USP L9 methods

• 5 µm particles for improved efficiency and resolution

• Narrow-bore format minimizes solvent consumption

• Fast analysis with 50 mm column length

• High-purity silica support

Typical Applications

• USP L9 methods

• Ion exchange chromatography

• Organic acid analysis

• Inorganic anion separations

• Biomolecule analysis

• LC-MS compatible workflows

• Method development

• Quality control testing

Specifications

• Product Type: HPLC Column

• Stationary Phase: IEX SAX

• USP Classification: L9

• Chromatography Mode: Strong Anion Exchange

• Functional Group: Quaternary Amine

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 50 mm

• Length: 50 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for ion exchange chromatography

• Retains negatively charged analytes

• Quaternary amine groups remain charged across a broad pH range

• Narrow-bore format requires a system capable of reliable low-flow operation

• Higher efficiency than comparable 10 µm ion exchange columns

• Reduced solvent consumption compared to 4.6 mm ID columns

• Excellent alternative for many USP L9 methods

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