Catalog Number - 67586-25P

Cogent IEX SCX is a strong cation exchange (SCX) HPLC stationary phase manufactured using high-purity silica and sulfonic acid functional groups that maintain a permanent negative charge across a wide pH range. This strong cation exchange mechanism provides reliable retention and separation of positively charged analytes, making the column suitable for ion exchange chromatography methods involving basic compounds, amines, peptides, pharmaceuticals, and other cationic species. The stationary phase is an excellent alternative for USP L9 methods and applications requiring robust cation exchange selectivity.

67586-25P is a 250 mm x 4.6 mm ID analytical column packed with 5 µm SCX particles with a 100 Å pore size. The extended 250 mm length provides increased theoretical plate counts and maximum resolving power for complex ion exchange separations, impurity profiling, purification studies, and challenging analytical applications requiring enhanced separation of closely related cationic compounds. The 5 µm particle size delivers higher efficiency, sharper peaks, and improved chromatographic performance compared to larger particle packings while maintaining compatibility with conventional HPLC systems. Typical operating flow rates are approximately 1.0–2.0 mL/min.

This USP L9 column is particularly useful for the separation and purification of positively charged compounds, including basic pharmaceuticals, amines, peptides, alkaloids, and other ionic species. The combination of strong cation exchange functionality, high-efficiency 5 µm particles, and extended column length makes it ideal for applications requiring maximum chromatographic resolution and reproducible ion exchange performance.

Key Benefits

• Strong cation exchange (SCX) stationary phase

• Sulfonic acid functional groups

• Permanent negative surface charge

• Suitable alternative for USP L9 methods

• 5 µm particles for improved efficiency and resolution

• Maximum resolving power from 250 mm column length

• High chromatographic performance

• High-purity silica support

Typical Applications

• USP L9 methods

• Ion exchange chromatography

• Basic compound analysis

• Pharmaceutical analysis

• Amine separations

• Peptide analysis

• Impurity profiling

• Purification studies

Specifications

• Product Type: HPLC Column

• Stationary Phase: IEX SCX

• USP Classification: L9

• Chromatography Mode: Strong Cation Exchange

• Functional Group: Sulfonic Acid

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for ion exchange chromatography

• Retains positively charged analytes

• Sulfonic acid groups remain charged across a broad pH range

• Longer column length provides maximum chromatographic resolution

• Higher efficiency than comparable 10 µm ion exchange columns

• Compatible with conventional HPLC systems

• Excellent alternative for many USP L9 methods

• One of the most popular Cogent SCX column configurations

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