HPLC Column, IEX WCX, 5um, 2.1mm ID x 50mm Length, 100A. Cogent Brand. 1 EA.
Additional Information:
Cogent IEX WCX is a weak cation exchange (WCX) HPLC stationary phase designed for the retention and separation of positively charged compounds. Manufactured using high-purity silica and carboxylic acid functional groups, the stationary phase provides a negatively charged surface within its functional pH range of approximately 2.5 to 7, enabling reliable cation exchange selectivity for basic compounds and other positively charged analytes. This chemistry is widely used in ion exchange chromatography and serves as an excellent alternative for many USP L9 methods.
67591-05P-2 is a 50 mm x 2.1 mm ID analytical HPLC column packed with 5 µm WCX particles with a 100 Å pore size. The short 50 mm column length is ideal for rapid analyses, fast method development, and high-throughput workflows. The 2.1 mm ID format is particularly well suited for LC-MS applications and reduced solvent consumption. Typical flow rates are approximately 0.2–0.3 mL/min; however, standard HPLC systems must be capable of delivering reliable low-flow performance to ensure accurate and reproducible chromatographic results.
This weak cation exchange column is particularly useful for the analysis and purification of basic pharmaceuticals, amines, peptides, proteins, and other positively charged compounds. The combination of selective WCX functionality, high-purity silica, and efficient 5 µm particles provides dependable chromatographic performance for challenging cation exchange applications while offering the advantages of lower solvent usage and enhanced LC-MS compatibility.
Key Benefits
Weak cation exchange (WCX) stationary phase
Carboxylic acid functional groups
Suitable for positively charged analytes
Alternative for many USP L9 methods
High-purity silica support
5 µm particles for efficient separations
Fast analysis capability with 50 mm column length
Reduced solvent consumption with 2.1 mm ID format
Well suited for LC-MS applications
Typical Applications
Ion exchange chromatography
Cation separations
Basic compound analysis
Pharmaceutical analysis
Amine separations
Peptide analysis
Protein analysis
USP L9 methods
LC-MS methods
Method development
Purification of positively charged compounds
Specifications
Product Type: HPLC Column
Stationary Phase: IEX WCX
USP Classification: L9
Chromatography Mode: Weak Cation Exchange
Functional Group: Carboxylic Acid
Particle Size: 5 µm
Pore Size: 100 Å
Column Dimensions: 2.1 mm ID x 50 mm
Length: 50 mm
Recommended Flow Rate: 0.2–0.3 mL/min
Brand: Cogent
Important Notes
Designed for weak cation exchange chromatography
Retains positively charged analytes
Functional charged range approximately pH 2.5–7
Suitable for many USP L9 applications
High-purity silica provides reliable chromatographic performance
Short column length supports rapid analyses and high-throughput workflows
Excellent choice for LC-MS applications
Standard HPLC systems must be capable of reliable low-flow operation for use with 2.1 mm ID columns