HPLC Column, RP C4 Butyl, Wide Pore, 10um, 4.0mm ID x 100mm Length, 300A. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent RP C4 Butyl Wide Pore is a reversed-phase HPLC stationary phase manufactured using high-purity silica with traditional butyl (C4) ligand bonding and complete double endcapping. Classified as USP L26, this stationary phase provides moderate hydrophobic retention while offering enhanced accessibility for larger molecules through its 300 Å pore structure. The C4 chemistry is particularly well suited for proteins, peptides, enzymes, and other biomolecules that benefit from efficient mass transfer and reduced retention compared to more hydrophobic reversed-phase columns.
68004-10P-4-3M is a 100 mm x 4.0 mm ID analytical HPLC column packed with 10 µm RP C4 Butyl Wide Pore particles featuring a 300 Å pore size. The 100 mm column length provides an ideal balance of chromatographic resolution and analysis time for routine bioanalytical methods, protein characterization, and peptide separations. The larger 10 µm particles allow operation at higher flow rates with lower backpressure, while the 4.0 mm ID format is commonly selected for legacy methods and conventional HPLC systems and typically operates at approximately 0.8–1.5 mL/min.
This USP L26 column is an excellent choice for the separation and characterization of proteins, peptides, enzymes, and other large hydrophobic or neutral compounds. The combination of fully endcapped C4 chemistry, high-purity silica, wide pore architecture, and larger particle size provides excellent analyte recovery and reproducible chromatographic performance for demanding bioanalysis and biomolecule research applications. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
RP C4 Butyl Wide Pore stationary phase
USP L26 classification
Fully double endcapped chemistry
Traditional butyl (C4) bonded phase
300 Å pore structure optimized for large molecules
High-purity silica support
10 µm particles for lower operating backpressure
Suitable for higher flow rates
Excellent for protein and peptide separations
Ideal for bioanalytical methods
Typical Applications
Reversed-phase HPLC
USP L26 methods
Protein analysis
Peptide analysis
Enzyme characterization
Bioanalytical methods
Biomolecule separations
Method development
Quality control testing
Large molecule analysis
Specifications
Product Type: HPLC Column
Stationary Phase: RP C4 Butyl Wide Pore
USP Classification: L26
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 4.0 mm ID x 100 mm
Length: 100 mm
Recommended Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Special Order Item Information
This item is a Special Order Item manufactured specifically to customer requirements.
Special Order Items cannot be canceled or returned once an order has been placed.
Manufactured upon receipt of order.
May have extended lead times compared to stocked products.
Important Notes
Designed for reversed-phase chromatography of proteins and other large molecules
Wide pore structure improves analyte access to the stationary phase
Provides less retention than comparable C8 columns
Larger particle size reduces operating backpressure
Excellent choice for bioanalytical and biomolecule characterization applications
Compatible with conventional HPLC systems
Alternative for many USP L26 methods
Special Order Item