Prep HPLC Column, RP C4, Wide Pore, 10um, 300A. 21.2mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C4 Butyl Wide Pore is a reversed-phase HPLC stationary phase manufactured using high-purity silica with traditional butyl (C4) ligand bonding and complete double endcapping. The 300 Å pore structure is specifically designed to provide improved accessibility for large biomolecules, making this stationary phase an excellent choice for proteins, peptides, enzymes, and other high-molecular-weight compounds. The moderate hydrophobicity of the C4 ligand promotes effective retention while maintaining excellent recovery during preparative purification workflows.
68004-P21-2503M is a preparative HPLC column with dimensions of 21.2 mm ID x 250 mm length, packed with 10 µm RP C4 Butyl Wide Pore particles featuring a 300 Å pore size. The large internal diameter is designed for high sample loading and fraction collection, while the extended 250 mm length provides maximum resolving power and peak capacity for demanding biomolecule purifications. Typical flow rates are approximately 15–40 mL/min depending on mobile phase composition, analyte properties, and system capabilities.
This column is specifically intended for preparative purification of proteins, peptides, enzymes, and other large hydrophobic or neutral biomolecules. The combination of wide pore architecture, fully endcapped C4 chemistry, and preparative dimensions provides excellent analyte recovery, loading capacity, and reproducible purification performance. The longer column length is particularly beneficial when separating closely related biomolecules, improving purity, or optimizing large-scale purification methods. A specialized preparative HPLC system capable of higher flow rates, larger injection volumes, and fraction collection is required. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
RP C4 Butyl Wide Pore stationary phase
300 Å pore size optimized for biomolecules
Fully double endcapped chemistry
Traditional butyl (C4) bonded phase
Designed for preparative HPLC purification
Excellent recovery of proteins and peptides
High sample loading capacity
10 µm particles for lower operating backpressure
Suitable for higher flow rates
Maximum resolution with 250 mm column length
Typical Applications
Preparative HPLC purification
Protein purification
Peptide purification
Enzyme purification
Biomolecule isolation
Biopharmaceutical research
Biotechnology research
Process development
Scale-up studies
Fraction collection
Specifications
Product Type: Preparative HPLC Column
Stationary Phase: RP C4 Butyl Wide Pore
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 21.2 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 15–40 mL/min
Brand: Cogent
Special Order Item Information
Important Notes
Designed for purification rather than routine analytical HPLC
Intended for collection and isolation of purified biomolecules
Optimized for proteins, peptides, enzymes, and other large molecules
Provides substantially higher sample loading capacity than analytical columns
Requires a preparative HPLC system capable of higher flow rates and fraction collection
Wide pore structure improves accessibility and recovery of biomolecules
Ideal for process development, scale-up studies, and production-support purification
Special Order Item