Process HPLC Column, RP C4, Wide Pore, 10um, 300A. 50mm ID x 50mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C4 Butyl Wide Pore is a reversed-phase HPLC stationary phase manufactured using high-purity silica with traditional butyl (C4) ligand bonding and complete double endcapping. The 300 Å pore structure is specifically designed to improve accessibility for proteins, peptides, enzymes, and other large biomolecules, making it an excellent choice for process-scale purification applications where high recovery, loading capacity, and reproducible performance are critical.
68004-P50-50-3M is a process HPLC column with dimensions of 50 mm ID x 50 mm length, packed with 10 µm RP C4 Butyl Wide Pore particles featuring a 300 Å pore size. The large internal diameter supports substantial sample loading and high-throughput purification, while the short 50 mm length is optimized for rapid purification cycles, process development, and manufacturing method screening. Typical flow rates are approximately 80–200 mL/min depending on system capabilities, mobile phase conditions, and process requirements.
This column is specifically intended for in-process purification of proteins, peptides, enzymes, and other large hydrophobic or neutral biomolecules. The combination of wide pore architecture, fully endcapped C4 chemistry, and process-scale dimensions provides excellent analyte recovery and purification efficiency. The short column format is particularly beneficial when throughput and cycle time are prioritized over maximum chromatographic resolution. A specialized process-scale HPLC system capable of high flow rates, large injection volumes, and fraction collection is required. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
RP C4 Butyl Wide Pore stationary phase
300 Å pore size optimized for biomolecules
Fully double endcapped chemistry
Traditional butyl (C4) bonded phase
Designed for process-scale purification
Excellent recovery of proteins and peptides
High sample loading capacity
10 µm particles for lower operating backpressure
Suitable for high flow rates
Fast purification cycles with 50 mm length
Typical Applications
Process-scale biomolecule purification
Protein purification
Peptide purification
Enzyme purification
Biopharmaceutical production support
Biotechnology research
Process development
Manufacturing method optimization
Production workflows
Fraction collection
Specifications
Product Type: Process HPLC Column
Stationary Phase: RP C4 Butyl Wide Pore
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 50 mm ID x 50 mm
Length: 50 mm
Typical Flow Rate: 80–200 mL/min
Brand: Cogent
Special Order Item Information
Important Notes
Designed for purification rather than routine analytical HPLC
Intended for collection and isolation of purified biomolecules
Optimized for proteins, peptides, enzymes, and other large molecules
Requires a process-scale HPLC system capable of high flow rates and fraction collection
Wide pore structure improves accessibility and recovery of biomolecules
Short column length supports rapid purification and process optimization workflows
Special Order Item