Semi Prep HPLC Column, RP C4 Wide Pore, 10um, 300A. 10mm ID x 50mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C4 Butyl Wide Pore is a reversed-phase HPLC stationary phase manufactured using high-purity silica with traditional butyl (C4) ligand bonding and complete double endcapping. The 300 Å pore structure is specifically designed to improve accessibility for proteins, peptides, enzymes, and other large biomolecules, making it an excellent choice for semi-preparative purification applications where analyte recovery and loading capacity are important considerations.
68004-SP50-3M is a semi-preparative HPLC column with dimensions of 10 mm ID x 50 mm length, packed with 10 µm RP C4 Butyl Wide Pore particles featuring a 300 Å pore size. The larger internal diameter provides substantially greater loading capacity than analytical columns, while the short 50 mm length is optimized for rapid purification cycles, method development, and process screening. The 10 µm particle size supports higher flow rates with lower operating backpressure. Typical flow rates are approximately 3–8 mL/min depending on mobile phase conditions, analyte properties, and system capabilities.
This column is specifically intended for semi-preparative purification of proteins, peptides, enzymes, and other large hydrophobic or neutral biomolecules. The combination of wide pore architecture, fully endcapped C4 chemistry, and semi-preparative dimensions provides excellent analyte recovery and reproducible purification performance. The short column format is particularly useful when throughput and cycle time are prioritized over maximum chromatographic resolution. Applications include protein isolation, peptide purification, biotechnology research, biopharmaceutical development, process development, and method scale-up. A specialized HPLC system capable of semi-preparative flow rates, larger injection volumes, and fraction collection is required. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
RP C4 Butyl Wide Pore stationary phase
300 Å pore size optimized for biomolecules
Fully double endcapped chemistry
Traditional butyl (C4) bonded phase
Designed for semi-preparative HPLC purification
Increased sample loading capacity
Excellent recovery of proteins and peptides
10 µm particles for lower operating backpressure
Suitable for higher flow rates
Fast purification cycles with 50 mm length
Typical Applications
Semi-preparative HPLC purification
Protein purification
Peptide purification
Enzyme purification
Biomolecule isolation
Biotechnology research
Biopharmaceutical research
Process development
Method scale-up
Fraction collection
Specifications
Product Type: Semi-Preparative HPLC Column
Stationary Phase: RP C4 Butyl Wide Pore
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 10 mm ID x 50 mm
Length: 50 mm
Typical Flow Rate: 3–8 mL/min
Brand: Cogent
Special Order Item Information
Important Notes
Designed for purification rather than routine analytical HPLC
Optimized for proteins, peptides, enzymes, and other large biomolecules
Provides higher loading capacity than analytical columns
Requires a semi-preparative HPLC system capable of appropriate flow rates and fraction collection
Wide pore structure improves accessibility and recovery of biomolecules
Short column length supports rapid purification and method development workflows
Special Order Item