Prep HPLC Column, RP C8 Octyl, Wide Pore, 10um, 300A. 21.2mm ID x 150mm Length. Cogent Brand. 1 EA.

Additional Information:

Cogent RP C8 Octyl Wide Pore is a reversed-phase preparative HPLC stationary phase manufactured using high-purity silica with traditional octyl (C8) silane bonding and complete double endcapping. The 300 Å pore structure is specifically designed to improve accessibility for proteins, peptides, and other large biomolecules while maintaining the moderate hydrophobicity and selectivity characteristic of C8 chemistry. This stationary phase is an excellent choice for preparative purification workflows where recovery, loading capacity, and efficient fraction collection are critical.

68008-P21-1503M is a preparative HPLC column with dimensions of 21.2 mm ID x 150 mm length, packed with 10 µm RP C8 Octyl Wide Pore particles featuring a 300 Å pore size. The large internal diameter provides substantial sample loading capacity, while the 150 mm length offers enhanced resolving power compared to shorter preparative columns. The 10 µm particle size supports higher flow rates with lower operating backpressure, making this column well suited for purification, process development, and preparative scale-up workflows. Typical operating flow rates are approximately 15–40 mL/min depending on mobile phase composition, sample load, and system capabilities.

Designed for purification and fraction collection rather than routine analytical HPLC, this column is particularly useful for proteins, peptides, enzymes, and other large hydrophobic or neutral biomolecules. Applications include biopharmaceutical research, biotechnology development, protein purification, peptide isolation, process development, and scale-up purification. Analytical methods are typically developed on analytical columns before transfer and optimization on preparative columns such as this one. A specialized preparative HPLC system capable of higher flow rates, larger injection volumes, and fraction collection is required. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.

Key Benefits

  • RP C8 Octyl Wide Pore stationary phase

  • 300 Å pore size optimized for biomolecules

  • Fully double endcapped chemistry

  • Traditional octyl (C8) bonded phase

  • Designed for preparative HPLC purification

  • High sample loading capacity

  • Excellent for proteins and peptides

  • 10 µm particles for lower operating backpressure

  • Suitable for higher preparative flow rates

  • Enhanced resolution with 150 mm length

Typical Applications

  • Preparative HPLC purification

  • Protein purification

  • Peptide purification

  • Enzyme purification

  • Biomolecule isolation

  • Biopharmaceutical research

  • Biotechnology research

  • Process development

  • Method scale-up

  • Fraction collection

Specifications

  • Product Type: Preparative HPLC Column

  • Stationary Phase: RP C8 Octyl Wide Pore

  • Chromatography Mode: Reversed Phase

  • Bonded Phase: Octyl (C8)

  • Endcapping: Fully Double Endcapped

  • Particle Size: 10 µm

  • Pore Size: 300 Å

  • Column Dimensions: 21.2 mm ID x 150 mm

  • Length: 150 mm

  • Typical Flow Rate: 15–40 mL/min

  • Brand: Cogent

Special Order Item Information

Important Notes

  • Intended for purification and fraction collection rather than routine analytical HPLC

  • Optimized for proteins, peptides, enzymes, and other large biomolecules

  • High loading capacity supports preparative-scale isolations

  • Analytical methods are often developed on analytical columns before transfer to preparative columns

  • Requires a preparative HPLC system capable of higher flow rates and fraction collection

  • Special Order Item


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