Process HPLC Column, RP C8 Octyl, Wide Pore, 10um, 300A. 50mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C8 Octyl Wide Pore is a reversed-phase process HPLC stationary phase manufactured using high-purity 300 Å silica with traditional octyl (C8) silane bonding and complete double endcapping. The wide pore structure is specifically designed to improve accessibility for proteins, peptides, and other large biomolecules while maintaining the moderate hydrophobicity and selectivity characteristic of C8 chemistry. This stationary phase is well suited for process-scale purification workflows where high recovery, efficient fraction collection, and loading capacity are critical.
68008-P50-2503M is a process HPLC column with dimensions of 50 mm ID x 250 mm length, packed with 10 µm RP C8 Octyl Wide Pore particles featuring a 300 Å pore size. The extra-large internal diameter provides exceptionally high sample loading capacity for production-scale purification, while the 250 mm length delivers maximum resolving power and peak capacity for demanding biomolecule separations. The 10 µm particle size supports high flow rates with lower operating backpressure, making this column well suited for process development, pilot-scale production, and manufacturing applications. Typical operating flow rates are approximately 80–200 mL/min depending on sample load, mobile phase composition, and system capabilities.
Designed for purification and fraction collection rather than routine analytical HPLC, this column is particularly useful for proteins, peptides, enzymes, and other large biomolecules. The extended column length can significantly improve separation of closely related biomolecules and help achieve higher purity during process-scale isolations. Analytical methods are typically developed and optimized on analytical columns before transfer to preparative and process-scale columns such as this one. A specialized process HPLC system capable of high flow rates, large injection volumes, and large-scale fraction collection is required. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
RP C8 Octyl Wide Pore stationary phase
300 Å pore size optimized for biomolecules
Fully double endcapped chemistry
Traditional octyl (C8) bonded phase
Designed for process-scale HPLC purification
Exceptionally high sample loading capacity
Excellent for proteins and peptides
10 µm particles for lower operating backpressure
Suitable for high process flow rates
Maximum resolution with 250 mm length
Typical Applications
Process HPLC purification
Protein purification
Peptide purification
Enzyme purification
Biomolecule isolation
Biopharmaceutical manufacturing
Biotechnology production
Process development
Pilot plant operations
Large-scale fraction collection
Specifications
Product Type: Process HPLC Column
Stationary Phase: RP C8 Octyl Wide Pore
Chromatography Mode: Reversed Phase
Bonded Phase: Octyl (C8)
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 50 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 80–200 mL/min
Brand: Cogent
Special Order Item Information
Important Notes
Intended for purification and fraction collection rather than routine analytical HPLC
Optimized for proteins, peptides, enzymes, and other large biomolecules
Exceptionally high loading capacity supports process-scale isolations
Extended column length can improve separation of closely related biomolecules
Analytical methods are often developed on analytical columns before transfer to preparative and process columns
Requires a process HPLC system capable of high flow rates and large-scale fraction collection
Special Order Item