Catalog Number - 68018-P21-250

The 68018‑P21‑250 Cogent RP C18 Preparative HPLC Column is engineered for maximum separation performance in high‑capacity purification workflows, utilizing 10 µm particles and fully double end‑capped C18 bonding to deliver reliable retention, high loading capacity, and durable chromatographic performance.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, this stationary phase provides:

• Complete end‑capping to minimize silanol interactions

• Improved peak symmetry for polar and basic analytes

• Consistent and reproducible purification results

This column is ideal as a USP L1 alternate and as a high‑performance platform for preparative method development and production‑scale purification workflows.

250 mm Length – Maximum Separation & Highest Purity

• Highest resolving power in the 21.2 mm RP C18 (10 µm) range

• Maximizes peak capacity for highly complex mixtures

• Ideal for closely related compounds and difficult separations

• Provides extended retention for hydrophobic analytes

• Best suited for final purification and highest‑purity isolation

This configuration is selected when shorter columns cannot achieve required resolution or purity.

21.2 mm ID – High‑Capacity Preparative Scale

• Provides very high sample loading capacity

• Designed for large‑scale purification and batch processing

• Supports high‑flow preparative chromatography workflows

Typical Flow Rate Range

• ~10–30 mL/min (system dependent)

• Optimize based on instrument capability and purification goals

✅ Designed for preparative HPLC systems capable of high flow rates and large injection volumes

Flow Rate & System Considerations (21.2 mm ID, 10 µm)

• Requires systems capable of:

  • High flow rates

  • Large injection volumes

• Compatible with:

  • Preparative pumps

  • High‑volume injection systems

  • Fraction collection systems

⚠️ Not intended for analytical or semi‑prep HPLC systems

Why Choose RP C18 (10 µm, Preparative Scale)

Maximum Resolution for Final Purification

• Ideal for final purification of complex mixtures

• Maximizes separation efficiency and product purity

• Supports production‑scale purification workflows

Reliable Hydrophobic Retention

• Strong reversed‑phase retention for hydrophobic and neutral compounds

• Excellent for long‑chain fatty acids

• Provides greater retention than C8 phases

Method Development & USP Compatibility

• Suitable for USP L1 methods

• Reliable for validated and regulated workflows

• Supports method transfer and scale‑up consistency

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances performance for challenging analytes

High Purity Silica

• Consistent chromatographic performance

• Reduced variability across runs and batches

Durable & Cost‑Efficient

• Long column lifetime

• Designed for high‑load purification workflows

• Reduced maintenance requirements

Typical Applications

• Preparative purification

• High‑purity compound isolation

• Complex mixture separations

• Pharmaceutical and QC workflows

• Pilot‑scale to production purification

• USP L1 reversed‑phase methods

Important Usage Notes

• Designed for preparative HPLC systems (21.2 mm ID)

• Requires high‑flow capability and large sample loading volumes

• Ideal for maximum‑resolution and high‑purity workflows

• Optimize for resolution vs throughput balance

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.