Prep HPLC Column, RP C18, Wide Pore, 10um, 300A. 21.2mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Info:
The 68018‑P21‑250‑3M Cogent RP C18 Wide Pore Preparative HPLC Column is engineered for maximum‑resolution biomolecule purification and production‑scale separations, combining 10 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, very high loading capacity, and durable chromatographic performance.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Consistent and reproducible purification results
The 300 Å pore size enables large biomolecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å media.
This column is ideal as a USP L1 alternate for large‑molecule applications and as a high‑performance platform for pilot‑scale and production purification workflows.
250 mm Length – Maximum Separation & Highest Purity
Highest resolving power in the 21.2 mm wide‑pore C18 range
Maximizes peak capacity for highly complex biomolecule mixtures
Ideal for proteins, peptides, and closely related macromolecules
Provides extended retention and improved separation of difficult analytes
Best suited for final purification and highest‑purity isolation
This configuration is selected when shorter columns cannot meet resolution or purity requirements.
21.2 mm ID – Production‑Scale Loading Capacity
Provides very high sample loading capacity
Designed for large‑scale biomolecule purification and batch production
Supports high‑flow preparative chromatography workflows
Typical Flow Rate Range
~10–30 mL/min (system dependent)
Optimize based on instrument capability and purification requirements
✅ Designed for preparative HPLC systems capable of high flow rates and large injection volumes
Flow Rate & System Considerations (21.2 mm ID, 10 µm, 300 Å)
Requires systems capable of:
High flow rates
Large injection volumes
Compatible with:
Preparative pumps
High‑volume injection systems
Fraction collection systems
⚠️ Not intended for analytical or semi‑prep HPLC systems
Why Choose Wide Pore (300 Å) C18 (10 µm, Preparative)
Designed for Biomolecules
Enables purification of:
Proteins
Peptides
Large hydrophobic compounds
Eliminates mass transfer limitations of smaller pore structures
Maximum‑Resolution Purification Platform
Ideal for final production‑scale purification workflows
Maximizes separation efficiency and product purity
Supports complex biomolecule separations
Scalable Workflow Integration
Predictable selectivity from analytical → semi‑prep → prep → production
Supports efficient process development and scale‑up
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances stability for biomolecule separations
High Purity Silica
Consistent chromatographic performance
Reduced variability across runs and batches
Durable & Cost‑Efficient
Long column lifetime
Designed for high‑load production workflows
Reduced maintenance and replacement frequency
Typical Applications
Production‑scale biomolecule purification
Protein and peptide separations
High‑purity compound isolation
Complex biomolecule mixtures
Pharmaceutical manufacturing and QC
USP L1 wide‑pore reversed‑phase method
Important Usage Notes
Designed for 300 Å wide‑pore biomolecule applications
Requires preparative HPLC system capability (21.2 mm ID)
Ideal for maximum‑resolution, high‑purity production workflows
Optimize for resolution vs throughput balance
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable