Catalog Number - 68018-P30-PG1-3

The 68018‑P30‑PG1‑3 Cogent RP C18 Wide Pore Prep Guard Column Cartridge is engineered to protect high‑value preparative HPLC columns operating at production scale, extending column life while maintaining consistent chromatographic performance.

Packed with 10 µm Cogent RP C18™ Wide Pore stationary phase (300 Å) and manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, this cartridge is fully double end‑capped to provide:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar analytes

• Reliable and reproducible chromatographic protection

Constructed in a stainless steel housing with stainless steel frits, this cartridge is built for durability in high‑flow, high‑load production environments.

Purpose of Guard Columns

• Protect preparative columns from:

  • Particulate contamination

  • Strongly retained biomolecules

  • Matrix buildup and fouling

• Extend column lifetime

• Maintain consistent retention and peak shape

• Reduce downtime and replacement costs

300 Å Wide Pore – Biomolecule Protection

• Designed specifically for large molecular weight analytes

• Ideal for:

  • Proteins

  • Peptides

  • Macromolecules

• Prevents:

  • Restricted diffusion effects

  • Pore blocking associated with 100 Å media

10 µm Guard Media – Production‑Scale Compatibility

• Matches 10 µm preparative column packing

• Supports very high loading and flow workflows

• Ideal for prep‑scale and production chromatography systems

✅ Maintains consistent selectivity across full scale‑up workflows

30 mm ID – Production‑Scale Systems

• Designed for 30 mm ID preparative HPLC columns

• Supports very high flow rates and large injection volumes

• Ideal for pilot‑scale and full production purification workflows

⚠️ Requires a 30 mm ID prep guard column holder system

Why Choose Wide Pore Guard Cartridges (10 µm, 300 Å, 30 mm)

Matched Chemistry for Biomolecules

• Same wide‑pore C18 chemistry as the primary column

• Maintains selectivity and chromatographic behavior

• Prevents unintended method changes

Enhanced Biomolecule Protection

• Captures strongly retained large molecules before reaching the column

• Reduces column fouling and degradation

Production‑Scale Alignment

• Designed for high‑load purification and manufacturing workflows

• Supports consistent performance across process scale‑up

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances stability for biomolecule separations

High Purity Silica

• Reliable cartridge‑to‑cartridge consistency

• Reproducible protection across extended runs

Durable Construction

• Stainless steel housing and frits

• Designed for high‑flow production systems

Typical Applications

• Production‑scale biomolecule purification

• Protein and peptide separations

• Preparative column protection

• Method development and process scale‑up

• Pharmaceutical manufacturing and QC

• USP L1 wide‑pore reversed‑phase methods

Important Usage Notes

• Designed for 300 Å wide‑pore biomolecule applications

• Must be used with a compatible 30 mm prep guard column holder

• Replace regularly to maintain optimal column performance

• Essential for protecting high‑value production columns

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.